EasySep™ Direct Human Basophil Isolation Kit

Immunomagnetic negative selection from whole blood kit
概要
The EasySep™ Direct Human Basophil Isolation Kit isolates functional, highly purified basophils directly from human whole blood by immunomagnetic negative selection. No lysis, density gradient centrifugation, or other processing steps are required and isolated cells are immediately available for flow cytometry, functional assays, culture, and other downstream applications.
Advantages
• Effectively depletes RBCs without the need for density gradient centrifugation, sedimentation, or lysis
• Up to 98% purity of isolated cells
• Fast, easy-to-use and column-free
• Isolated cells are untouched
Components
  • EasySep™ Direct Human Basophil Isolation Kit (Catalog #19667)
    • EasySep™ Direct Human Basophil Isolation Cocktail, 2 x 2.5 mL
    • EasySep™ Direct RapidSpheres™ 50300, 4 x 2.5 mL
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
Subtype
Cell Isolation Kits
Cell Type
Granulocytes and Subsets
Species
Human
Sample Source
Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology
数据及文献

Data

Figure 1. Typical EasySep™ Direct Human Basophil Isolation Profile

Starting with human whole blood from normal healthy donors, the typical basophil (CD123+IgE+) content of the non-lysed final isolated fraction is 97.3 ± 1.1% (gated on CD45). In the above example, the basophil content of the lysed whole blood start sample and the non-lysed final isolated fraction is 0.73% and 96.0% (gated on CD45), respectively, or 0.68% and 89.8% (not gated on CD45), respectively.

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