EasySep™ Direct HLA Crossmatch T Cell Isolation Kit

Immunomagnetic negative selection from whole blood, spleen or lymph node kit
概要
The EasySep™ Direct HLA Crossmatch T Cell Isolation Kit can be used to isolate highly purified T cells directly from human whole blood, spleen or lymph nodes by immunomagnetic negative selection. This kit targets non-T cells for removal with antibodies recognizing specific surface markers. Unwanted cells are labeled with antibodies and EasySep™ Direct RapidSpheres™, and separated using an EasySep™ magnet. Desired cells are simply collected into a new tube and are immediately available for downstream applications such as flow cytometry crossmatch.
Advantages
• > 99.9% RBC depletion without the need for density gradient centrifugation, sedimentation, or lysis
• Up to 99.9% purity of isolated cells
• Fast, easy-to-use, and column-free
• Isolated cells are untouched
Components
  • EasySep™ Direct HLA Crossmatch T Cell Isolation Kit (Catalog #19671)
    • EasySep™ Direct HLA Crossmatch T Cell Isolation Cocktail, 2 x 2.5 mL
    • EasySep™ Direct RapidSpheres™, 4 x 2.5 mL
  • EasySep™ Direct HLA Crossmatch T Cell Isolation Kit for RoboSep™ (Catalog #19671RF)
    • EasySep™ Direct HLA Crossmatch T Cell Isolation Cocktail, 2 x 2.5 mL
    • EasySep™ Direct RapidSpheres™, 4 x 2.5 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells
Species
Human
Sample Source
Lymph Node, Spleen, Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
HLA, Immunology
数据及文献

Data

Starting with human whole blood from normal healthy donors, the T cell (CD3+) content of the non-lysed final isolated fraction typically ranges from 89 - 99.9% (gated on CD45). In the above example, the T cell (CD3+) content of the lysed whole blood start sample and non-lysed final isolated fraction is 38.0% and 93.2% (gated on CD45), respectively.

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