EasySep™ Serology Whole Blood CD3 Positive Selection Kit

Immunomagnetic Positive Selection for HLA serology testing
概要
EasySep™ Serology Whole Blood CD3 Positive Selection Kit is designed to isolate highly purified CD3+ cells from fresh human whole blood by immunomagnetic positive selection. This kit targets human CD3+ cells for positive selection with an antibody recognizing the CD3 surface marker. Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated cells are immediately available for downstream applications such as HLA serology, flow cytometry, or DNA/RNA extraction.                                
Advantages
⦁ Fast and easy-to-use
⦁ Up to 99% purity
⦁ No columns required
⦁ Compatible with HLA serological applications
Components
  • EasySep™ Serology Whole Blood CD3 Positive Selection Kit (Catalog #18981)
    • EasySep™ Serology Whole Blood CD3 Positive Selection Cocktail, 2 x 0.65 mL
    • EasySep™ Releasable RapidSpheres™ 50201, 6 x 1 mL
  • RoboSep™ Serology Whole Blood CD3 Positive Selection Kit (Catalog #18981RF)
    • EasySep™ Serology Whole Blood CD3 Positive Selection Cocktail, 2 x 0.65 mL
    • EasySep™ Releasable RapidSpheres™ 50201, 6 x 1 mL
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Tips (Catalog #20125) x 2
Magnet Compatibility
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
细胞分选试剂盒
Cell Type
T细胞
Species
Sample Source
全血
Selection Method
正选
Application
细胞分离
Brand
EasySep, RoboSep
Area of Interest
HLA, 免疫学
数据及文献

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

数据

Using the EasySep™ Serology Whole Blood CD3 Positive Selection Kit on human whole blood, the CD19+ purities of the start and final isolated fractions are 25.4% and 98.7%, respectively (gated on CD45+).

Figure 1. EasySep™ Serology Whole Blood CD3 Positive Selection Kit

Starting with human whole blood, the CD3+ cell content of the isolated fraction typically ranges from 96.7 - 99.6% (note: as the CD3 antibody used for flow cytometry staining was fully blocked by the CD3 antibody used to isolate the cells, staining of T cells was assessed by labeling with anti-CD2; gated on CD45+). In the above example, the purities of the start and final isolated fractions are 25.4% and 98.7% (gated on CD45+), respectively.
NOTE: Red blood cells were removed by lysis prior to flow cytometry.
NOTE: The magnetic particles present on the surface of the isolated CD3+ cells will cause a large FSC/SSC shift when the samples are run on a flow cytometer. This will make it difficult to gate on a specific lymphocyte population based on FSC/SSC. Instead, we recommend first gating the cells on CD45 and examining the lymphocyte populations based on the CD45+ gate.


Top