StemSep™ Human CD14 Positive Selection Kit
Immunomagnetic column-based positive selection kit
概要
The StemSep™ Human CD14 Positive Selection Kit is designed to isolate CD14+ cells from fresh or previously frozen peripheral blood mononuclear cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD14 and dextran-coated magnetic particles. Labeled cells are separated using commercially available columns and StemSep™ magnets. The CD14 antigen is strongly expressed on monocytes and weakly expressed on granulocytes. It is also expresed on most tissue macrophages.
Components
- StemSep™ Human CD14 Positive Selection Kit (Catalog #14758)
- StemSep™ Human CD14 Positive Selection Cocktail, 1 mL
- StemSep™ Magnetic Colloid, 1.5 mL
Magnet Compatibility
• StemSep™ Magnet (Catalog #11030, 11050, 11060, 11070) or a magnet with the strength of at least 0.6 Tesla
• Commercially available positive selection columns
Subtype
Cell Isolation Kits
Cell Type
Monocytes
Species
Human
Sample Source
PBMC
Selection Method
Positive
Application
Cell Isolation
Brand
StemSep
Area of Interest
Immunology
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | StemSep™ Human CD14 Positive Selection Kit | 14758 | All | English |
| Manual | StemSep™ Human CD14 Positive Selection Kit | 14758 | All | English |
| Safety Data Sheet 1 | StemSep™ Human CD14 Positive Selection Kit | 14758 | All | English |
| Safety Data Sheet 2 | StemSep™ Human CD14 Positive Selection Kit | 14758 | All | English |
数据及文献
Data
Figure 1. Typical StemSep™ CD14 Selection Profile
The expected CD14+ content of the enriched fraction is 97.5 ± 0.5% with expected recoveries of 58 ± 9%.