RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Kit

Immunodensity negative selection cocktail
概要
The RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Kit is designed to pre-enrich hematopoietic progenitor cells from cord blood by negative selection. Unwanted cells are targeted for removal with antibody complexes recognizing CD3, CD11b, CD19 and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The hematopoietic progenitor cells are present as an enriched population at the interface between the plasma and the buoyant density medium.

Cells of interest can then be positively selected from the pre-enriched cell population using EasySep™ (e.g. EasySep™ Human CD34 Positive Selection).
Advantages
• Fast and easy-to-use
• Requires no special equipment or training
• Untouched, viable cells
• Can be combined with SepMate™ for consistent, high-throughput sample processing
Components
  • RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Kit (Catalog #15226)
    • RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail, 2 x 2.5 mL
  • RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail (Catalog #15266)
    • RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Cocktail, 10 x 2.5 mL
Magnet Compatibility
 
Subtype
Cell Isolation Kits
Cell Type
Hematopoietic Stem and Progenitor Cells
Species
Human
Sample Source
Cord Blood
Selection Method
Negative
Application
Cell Isolation
Brand
RosetteSep
Area of Interest
Immunology, Stem Cell Biology
数据及文献

Data

Typical RosetteSep™ Human Progenitor Cell Basic Preenrichment Profile

Figure 1.Typical RosetteSep™ Human Progenitor Cell Basic Pre-Enrichment Profile

Starting with fresh human cord blood, the CD34+CD45+ viable cell content of the pre-enriched fraction typically ranges from 2 - 16%

Top