RosetteSep™ Human ILC2 Enrichment Kit
Immunodensity negative selection cocktail
概要
The RosetteSep™ Human ILC2 Enrichment Cocktail is designed to enrich human ILC2s from whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-ILC2s and glycophorin A on red blood cells (RBCs). When centrifuged over a density gradient medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The ILC2 cells are present as a highly enriched population at the interface between the plasma and the density gradient medium.
Advantages
• Fast and easy-to-use
• Requires no special equipment or training
• Untouched, viable cells
• Can be combined with SepMate™ for consistent, high-throughput sample processing
• Facilitates rapid flow sorting of ILC2s
• Requires no special equipment or training
• Untouched, viable cells
• Can be combined with SepMate™ for consistent, high-throughput sample processing
• Facilitates rapid flow sorting of ILC2s
Components
- RosetteSep™ Human ILC2 Enrichment Kit (Catalog #15382)
- RosetteSep™ Human ILC2 Enrichment Cocktail, 5 x 2 mL
Subtype
Cell Isolation Kits
Cell Type
Innate Lymphoid Cells
Species
Human
Sample Source
Whole Blood
Selection Method
Negative
Brand
RosetteSep
Area of Interest
Immunology
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | RosetteSep™ Human ILC2 Enrichment Kit | 15382 | All | English |
| Safety Data Sheet | RosetteSep™ Human ILC2 Enrichment Kit | 15382 | All | English |
数据及文献
Data
Starting with fresh whole blood, the ILC2 content (Lin-CD45+CD294+CD127+CD161+) of the enriched fraction typically ranges from 0.44 - 53%. In the above example, the percentage of ILC2s in the start and final enriched fractions are 0.02% and 10.1%, respectively.