RosetteSep™ Human Cord Blood Debulking Cocktail

Immunodensity depletion cocktail
概要
The RosetteSep™ Human Cord Blood Debulking Cocktail is designed to deplete lineage positive cells from fresh cord blood. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing CD2, CD14, CD19, CD66b and glycophorin A on red blood cells (RBCs). When centrifuged over a buoyant density medium such as Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The hematopoietic stem and progenitor cells are present as a pre-enriched population at the interface between the plasma and the buoyant density medium.
Advantages
• Fast and easy-to-use
• Requires no special equipment or training
• Untouched, viable cells
• Can be combined with SepMate™ for consistent, high-throughput sample processing
Components
  • RosetteSep™ Human Cord Blood Debulking Cocktail (Catalog #15126)
    • RosetteSep™ Human Debulking Cocktail, 2 mL
  • RosetteSep™ Human Cord Blood Debulking Cocktail (Catalog #15166)
    • RosetteSep™ Human Debulking Cocktail, 5 x 2 mL
Subtype
Cell Isolation Kits
Cell Type
Hematopoietic Stem and Progenitor Cells
Species
Human
Sample Source
Cord Blood
Selection Method
Depletion
Application
Cell Isolation
Brand
RosetteSep
Area of Interest
Immunology, Stem Cell Biology
技术资料
Document Type 产品名称 Catalog # Lot # 语言
Product Information Sheet RosetteSep™ Human Cord Blood Debulking Cocktail 15126, 15166 All English
Safety Data Sheet RosetteSep™ Human Cord Blood Debulking Cocktail 15126 All English
数据及文献

Data

FACS Profile Results with RosetteSep™ De-Bulking Cocktail

Figure 1. FACS Profile Results with RosetteSep™ De-Bulking Cocktail

Starting with fresh cord blood, the enrichment of CD34+ cells is typically 5 ± 1%.

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