RosetteSep™ HLA B Cell Enrichment Cocktail

Immunodensity negative selection cocktail
概要
The RosetteSep™ HLA B Cell Enrichment Cocktail is designed to isolate B cells from whole blood by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-B cells and red blood cells (RBCs). When centrifuged over a buoyant density medium such as RosetteSep™ DM-L (Catalog #15705) or Lymphoprep™ (Catalog #07801), the unwanted cells pellet along with the RBCs. The purified B cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium. The desired cells are immediately ready for serology or flow cytometry crossmatch assays.

This product carries the CE mark and is available as a Class I in vitro diagnostic device in the European Union and Australia.
Advantages
• Fast and easy-to-use
• Requires no special equipment or training
• Isolated cells are untouched
Components
  • RosetteSep™ HLA B Cell Enrichment Cocktail (Catalog #15064HLA)
    • RosetteSep™ HLA B Cell Enrichment Cocktail, 5 x 2 mL
  • RosetteSep™ HLA B Cell Enrichment Cocktail (Catalog #15084HLA)
    • RosetteSep™ HLA B Cell Enrichment Cocktail, 20 x 2 mL
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Human
Sample Source
Buffy Coat, Whole Blood
Selection Method
Negative
Application
Cell Isolation, In Vitro Diagnostic
Brand
RosetteSep
Area of Interest
HLA, Immunology
数据及文献

Data

FACS Histogram Results with RosetteSep™ HLA B Cell Enrichment Kit

Figure 1. FACS Histogram Results with RosetteSep™ HLA B Cell Enrichment Kit

Starting with whole blood, the CD19+ cell content of the enriched fraction typically ranges from 59.3% - 97.2%.

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