RoboSep™-C Human CD4+ T Cell Isolation Kit
Immunomagnetic negative selection kit
概要
The RoboSep™-C Human CD4+ T Cell Isolation Kit is designed to isolate untouched and highly purified CD4+ T cells from fresh human peripheral blood leukopaks using RoboSep™-C (Catalog #100-0185 and #100-0186) for automated immunomagnetic negative selection.
This kit targets non-CD4+ T cells for removal with antibodies recognizing specific cell surface markers. Using RoboSep™-C in a fully automated protocol, unwanted cells are labeled with antibodies and magnetic particles, and separated without columns using a RoboSep™-C magnet. The desired untouched cells are washed and concentrated by RoboSep™-C and transferred to a final product bag. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or cryopreservation.
This kit targets non-CD4+ T cells for removal with antibodies recognizing specific cell surface markers. Using RoboSep™-C in a fully automated protocol, unwanted cells are labeled with antibodies and magnetic particles, and separated without columns using a RoboSep™-C magnet. The desired untouched cells are washed and concentrated by RoboSep™-C and transferred to a final product bag. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or cryopreservation.
Advantages
⦁ Fully automated large-scale isolation in a closed system
⦁ Up to 98% purity with high recovery
⦁ Untouched, viable cells
⦁ Up to 98% purity with high recovery
⦁ Untouched, viable cells
Components
- RoboSep™-C Human CD4+ T Cell Isolation Cocktail, 10 mL
- RoboSep™-C Dextran RapidSpheres™ 30106, 10 mL
Magnet Compatibility
• RoboSep™-C (Catalog #400-2274)
Subtype
Cell Isolation Kits
Cell Type
T Cells, T Cells, CD4+
Species
Human
Sample Source
Leukapheresis
Selection Method
Negative
Application
Cell Isolation
Brand
RoboSep
Area of Interest
Cell Therapy, Drug Discovery and Toxicity Testing, Immunology
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | RoboSep™-C Human CD4+ T Cell Isolation Kit | 100-0200 | All | English |
| Safety Data Sheet 1 | RoboSep™-C Human CD4+ T Cell Isolation Kit | 100-0200 | All | English |
| Safety Data Sheet 2 | RoboSep™-C Human CD4+ T Cell Isolation Kit | 100-0200 | All | English |
数据及文献
Data
Figure 1. Typical RoboSep™-C Human CD4+ T Cell Isolation Profile
Starting from a fresh human peripheral blood leukopak, the CD4+ T cell content (CD3+CD4+) of the isolated fraction is typically 95.7 ± 2.8% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 31.5% and 95.4%, respectively.