EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit

Immunomagnetic positive selection kit using particle release technology
概要
The EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Kit is designed to isolate CD271⁺ cells from human pluripotent stem cell (PSC)-derived neural crest cell culture by immunomagnetic positive selection.

Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated CD271+ cells, which are immediately available for downstream applications.

Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
Advantages
⦁ Highly purified human PSC-derived neural crest cells isolated in less than 20 minutes
⦁ No-wash removal of EasySep™ Releasable RapidSpheres™
⦁ Compatible with downstream differentiation protocols
Components
  • EasySep™ Release Human PSC-Derived Neural Crest Cell Positive Selection Cocktail, 2 x 1.0 mL
  • EasySep™ Isolation Cocktail Enhancer, 1.0 mL
  • EasySep™ Release Buffer (Concentrate), 3 x 1.0 mL
  • EasySep™ Releasable RapidSpheres™ 50201, 2 x 1.0 mL
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
Subtype
Cell Isolation Kits
Cell Type
Neural Cells, PSC-Derived
Species
Human
Sample Source
Other
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep
Area of Interest
Disease Modeling, Neuroscience
数据及文献

Data

A H9 cell-derived cell suspension assessed for CD271-PE. The the start and final isolated fractions are 74.9% and 93.8% pure.

Figure 1. Histograms of H9 cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry

Starting with a single-cell suspension of H9 cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).

A H7 cell-derived cell suspension assessed for CD271-PE. The the start and final isolated fractions are 47.3% and 90.8% pure.

Figure 2. Histograms of H7 cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry

Starting with a single-cell suspension of H7 cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).

A 1C cell-derived cell suspension assessed for CD271-PE. The the start and final isolated fractions are 18.9% and 86.7% pure.

Figure 3. Histograms of 1C cell suspensions assessed for CD271-phycoerythrin (PE) expression by flow cytometry

Starting with a single-cell suspension of 1C cells differentiated using STEMdiff™ Neural Crest Differentiation Kit (Catalog #08610), the CD271+ cell content of the isolated fractions is typically 92.9 ± 6.3% (mean ± SD using the purple EasySep™ Magnet).

Top