EasySep™ Rat CD4+ T Cell Isolation Kit

Immunomagnetic negative selection kit
概要
The EasySep™ Rat CD4+ T Cell Isolation Kit is designed to isolate CD4+ T cells from single-cell suspensions of splenocytes or lymph nodes or from whole blood by negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
Advantages
• Fast, easy-to-use and column-free
• Up to 98% purity
• Isolated cells are untouched
Components
  • EasySep™ Rat CD4+ T Cell Isolation Kit (Catalog #19642)
    • EasySep™ Rat CD4+ T Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 1 mL
  • RoboSep™ Rat CD4+ T Cell Isolation Kit (Catalog #19642RF)
    • EasySep™ Rat CD4+ T Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells, T Cells, CD4+
Species
Rat
Sample Source
Other, Spleen, Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology
数据及文献

Data

Figure 1. Typical EasySep™ Rat CD4+ T Cell Isolation Profile

Starting with rat splenocytes, the CD4+ T cell content (CD3+CD4+) of the isolated fraction is typically 97.8 ± 0.8% (mean ± SD using the purple EasySep™ Magnet with the optional additional separation).

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