EasySep™ Rat B Cell Isolation Kit

Immunomagnetic negative selection kit
概要
The EasySep™ Rat B Cell Isolation Kit is designed to isolate B cells from single-cell suspensions of splenocytes or lymph nodes or from whole blood by negative selection. The EasySep™ procedure involves labeling unwanted cells with antibody complexes and magnetic particles. The magnetically labeled cells are separated from the untouched desired cells by using an EasySep™ magnet and simply pouring or pipetting the desired cells into a new tube.
Advantages
• Fast, easy-to-use and column-free
• Up to 95% purity
• Isolated cells are untouched
Components
EasySep™ Rat B Cell Isolation Kit (Catalog #19644)
    • EasySep™ Rat B Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 1 mL
RoboSep™ Rat B Cell Isolation Kit (Catalog #19644RF)
    • EasySep™ Rat B Cell Isolation Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50102, 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• ”The BigEasy” EasySep™ Magnet (Catalog #18001)
• Easy50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells
Species
Rat
Sample Source
Other, Spleen, Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology
数据及文献

Data

19644 FACS

Starting with a single-cell suspension of rat splenocytes, the B cell content (CD45RA+CD3-) of the isolated fraction is typically 94.1 ± 3.8% (mean ± SD using the purple EasySep™ Magnet). In the example above, the purities of the start and final isolated fractions are 44.5% and 95.6%, respectively.

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