EasySep™ Mouse Pan-ILC Enrichment Kit

16-Minute cell isolation using Immunomagnetic negative selection
概要
This product is designed to enrich group 1, 2 and 3 innate lymphoid cells (ILC1, ILC2 and ILC3s) from single-cell suspensions of mouse tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-ILCs and streptavidin-coated magnetic particles (RapidSpheres™). Labeled cells are separated using an EasySep™ magnet without the use of columns. Unwanted cells remain in the tube as desired cells are simply poured off into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry and cell sorting.
Advantages
• Fast, easy-to-use and column-free
• Isolated cells are untouched
• Facilitates rapid flow sorting of ILCs
Components
  • EasySep™ Mouse Pan-ILC Enrichment Kit (Catalog #19875)
    • EasySep™ Mouse Pan-ILC Enrichment Cocktail, 0.5 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001, 1.0 mL
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• EasyPlate™ (Catalog #18102)
Subtype
Cell Isolation Kits
Cell Type
Innate Lymphoid Cells
Species
Mouse
Sample Source
Bone Marrow, Lung, Other
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology
技术资料
Document Type 产品名称 Catalog # Lot # 语言
Product Information Sheet EasySep™ Mouse Pan-ILC Enrichment Kit 19875 All English
Safety Data Sheet EasySep™ Mouse Pan-ILC Enrichment Kit 19875 All English
数据及文献

Data

Figure 1. Typical EasySep™ Mouse Pan-ILC Enrichment Profile

Starting with a naïve mouse lung single-cell suspension, the total ILC content (CD45+Lin-CD127+) of the enriched fraction typically ranges from 3.1 - 7.6%. In the above example, the percentages of ILCs in the start and final enriched fractions are 0.4% and 3.9% (or 0.4% and 6.8% of CD45+ cells), respectively. NOTE: The ILC content of the start fraction typically ranges from 0.3 - 0.6%.

Figure 2. Typical EasySep™ Mouse Pan-ILC Enrichment Profile from Mouse Lymph Node

Starting with a naïve mouse lymph node single-cell suspension, the total ILC content (CD45+Lin-CD127+) of the enriched fraction typically ranges from 21.1 - 45.2%. In the above example, the percentages of ILCs in the start and final enriched fractions are 0.3% and 17.6% (or 0.3% and 24.7% of CD45+ cells), respectively. NOTE: The ILC content of the start fraction typically ranges from 0.3 - 0.4%.

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