EasySep™ Mouse Pan-DC Enrichment Kit

Immunomagnetic negative selection cell isolation kit
概要
The EasySep™ Mouse Pan-DC Enrichment Kit is designed to isolate pan-dendritic cells (DCs) from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-pan-DCs and Tetrameric Antibody Complexes recognizing biotin and dextran-coated magnetic particles. Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.

For even faster cell isolations and greater recovery, we recommend the new EasySep™ Mouse Pan-DC Enrichment Kit II (19863) which isolates cells in just 13 minutes.
Advantages
• Fast, easy-to-use and column-free
• Up to 76% purity
• Isolated cells are untouched
Components
  • EasySep™ Mouse Pan-DC Enrichment Kit (Catalog #19763)
    • EasySep™ Mouse Pan-DC Enrichment Cocktail, 1.0 mL
    • EasySep™ Biotin Selection Cocktail, 2 x 1 mL
    • EasySep™ Magnetic Particles, 4 x 1 mL
  • RoboSep™ Mouse Pan-DC Enrichment Kit with Filter Tips (Catalog #19763RF)
    • EasySep™ Mouse Pan-DC Enrichment Cocktail, 1.0 mL
    • EasySep™ Biotin Selection Cocktail, 2 x 1 mL
    • EasySep™ Magnetic Particles, 4 x 1 mL
    • RoboSep™ Buffer (Catalog #20104) x 2
    • RoboSep™ Filter Tips (Catalog #20125) x 2
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Dendritic Cells
Species
Mouse
Sample Source
Spleen
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology
数据及文献

Data

Start: 3.61% Pan-dendritic Cells

Figure 1. Start: 3.61% Pan-dendritic Cells

Enriched Pan-Dendritic Cells obtained using EasySep™ Mouse pan-DC Enrichment Kit

Figure 2. Enriched: 67.8% Pan-dendritic Cells

The dendritic cell content of the enriched fraction is typically 65 ± 11%.

Top