EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit

18-Minute cell isolation kit using immunomagnetic negative selection
概要
The EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit is designed to isolate CD11b+Gr1+ myeloid-derived suppressor cells (MDSCs) from single-cell suspensions of splenocytes or other tissues by negative selection. Unwanted cells are targeted for removal with biotinylated antibodies directed against non-MDSCs and streptavidin-coated magnetic particles (RapidSpheres™). Labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube. Isolated cells are immediately available for downstream applications such as flow cytometry, cell sorting, and cell culture.
Advantages
• Fast, easy-to-use and column-free
• Up to 96% purity
• Untouched, viable cells
Components
  • EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit (Catalog #19867)
    • EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Cocktail, 0.5 mL
    • EasySep™ Streptavidin RapidSpheres™ 50001, 1.0 mL
    • EasySep™ Mouse FcR Blocker, 2 x 0.2 mL
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
Subtype
Cell Isolation Kits
Cell Type
Granulocytes and Subsets, Monocytes, Myeloid Cells
Species
Mouse
Sample Source
Bone Marrow, Other, Whole Blood
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep
Area of Interest
Immunology
数据及文献

Data

Figure 1. Typical EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit Profile from 4T1 Tumor-Bearing BALB/c Mouse Splenocytes

Starting with 4T1 tumor-bearing BALB/c mouse splenocytes, the MDSC content (CD11b+Gr1+) of the isolated fraction is typically 94.3 ± 2.1% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 20.7% and 94.7%, respectively.

Figure 2. Typical EasySep™ Mouse MDSC (CD11b+Gr1+) Isolation Kit Profile from Naïve C57BL/6 Mouse Splenocytes

Starting with naïve C57BL/6 mouse splenocytes, the CD11b+Gr1+ cell content of the isolated fraction is typically 86 ± 4.6% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final isolated fractions are 3.0% and 86.8%, respectively.

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