EasySep™ Mouse CD90.1 Positive Selection Kit

Immunomagnetic positive selection cell isolation kit
概要
The EasySep™ Mouse CD90.1 Positive Selection Kit is designed to isolate CD90.1+ (Thy1.1+) cells from single suspensions of splenocytes, lymph nodes and peripheral blood by positive selection. Desired cells are targeted with antibody complexes recognizing CD90.1 and dextran-coated magnetic particles. Labelled cells are separated using an EasySep™ magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.
Advantages
• Fast, and easy-to-use
• Up to 97% purity
• No columns required
• Isolated cells are not fluorochrome-labeled
Components
  • EasySep™ Mouse CD90.1 Positive Selection Kit (Catalog #18958)
    • EasySep™ Mouse CD90.1 Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
  • RoboSep™ Mouse CD90.1 Positive Selection Kit (Catalog #18958RF)
    • EasySep™ Mouse CD90.1 Positive Selection Cocktail, 1 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™- S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
T Cells
Species
Mouse
Sample Source
Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology
数据及文献

Data

FACS Histogram Results with EasySep™ Mouse CD90.1 Positive Selection Kit

Figure 1. FACS Histogram Results with EasySep™ Mouse CD90.1 Positive Selection Kit

Starting with mouse splenocytes, the CD90.1+ (Thy1.1+) cell content of the isolated fraction is typically 93.5 ± 3.9% (mean ± SD; using the purple EasySep™ Magnet).

FACS Histogram Results with EasySep™ Mouse CD90.1 Positive Selection Kit on Adoptive Lymphocyte Transfer Samples

Figure 2. FACS Histogram Results with EasySep™ Mouse CD90.1 Positive Selection Kit on Adoptive Lymphocyte Transfer Samples

Starting with mouse splenocytes from adoptive lymphocyte transfer samples, the CD90.1+ (Thy1.1+) cell content of the isolated fraction is typically 90.8 ± 1.5% (mean ± SD; using the purple EasySep™ Magnet).

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