EasySep™ Mouse CD11c Positive Selection Kit II

Immunomagnetic positive selection cell isolation kit
概要
The EasySep™ Mouse CD11c Positive Selection Kit II isolates highly purified CD11c+ cells from splenocytes or other tissues by immunomagnetic positive selection. Desired cells are targeted with antibodies and magnetic particles, and isolated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated cells are immediately ready for downstream applications such as flow cytometry, culture, and cell-based experiments.

This product replaces the EasySep™ Mouse CD11c Positive Selection Kit (Catalog #18758) for even faster cell isolations and does not result in the labeling of isolated cells with PE.
Advantages
• Fast and easy-to-use
• Up to 95% purity
• No columns required
• Isolated cells are not fluorochrome-labeled
Components
  • EasySep™ Mouse CD11c Positive Selection Kit II (Catalog #18780)
    • EasySep™ Mouse CD11c Positive Selection II Component A, 0.5 mL
    • EasySep™ Mouse CD11c Positive Selection II Component B, 0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
    • Rat Serum, 2 mL
    • Empty Vial for use with RoboSep™
  • RoboSep™ Mouse CD11c Positive Selection Kit II (Catalog #18780RF)
    • EasySep™ Mouse CD11c Positive Selection II Component A, 0.5 mL
    • EasySep™ Mouse CD11c Positive Selection II Component B, 0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
    • Rat Serum, 2 mL
    • Empty Vial for use with RoboSep™
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
  • EasySep™ Mouse CD11c Positive Selection Kit II with Spleen Dissociation Medium (Catalog #18781)
    • EasySep™ Mouse CD11c Positive Selection II Component A, 0.5 mL
    • EasySep™ Mouse CD11c Positive Selection II Component B, 0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
    • Rat Serum, 2 mL
    • Empty Vial for use with RoboSep™
    • Spleen Dissociation Medium (Catalog #07915)
  • RoboSep™ Mouse CD11c Positive Selection Kit II with Spleen Dissociation Medium (Catalog #18781RF)
    • EasySep™ Mouse CD11c Positive Selection II Component A, 0.5 mL
    • EasySep™ Mouse CD11c Positive Selection II Component B, 0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 2 x 1 mL
    • Rat Serum, 2 mL
    • Empty Vial for use with RoboSep™
    • Spleen Dissociation Medium (Catalog #07915)
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
Dendritic Cells, Granulocytes and Subsets, Monocytes, NK Cells
Species
Mouse
Sample Source
Bone Marrow, Other, Spleen
Selection Method
Positive
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology
技术资料
Document Type 产品名称 Catalog # Lot # 语言
Product Information Sheet EasySep™ Mouse CD11c Positive Selection Kit II 18780 All English
Product Information Sheet RoboSep™ Mouse CD11c Positive Selection Kit II 18780RF All English
Product Information Sheet EasySep™ Mouse CD11c Positive Selection Kit II with Spleen Dissociation Medium 18781 All English
Product Information Sheet RoboSep™ Mouse CD11c Positive Selection Kit II with Spleen Dissociation Medium 18781RF All English
Safety Data Sheet 1 EasySep™ Mouse CD11c Positive Selection Kit II 18780 All English
Safety Data Sheet 2 EasySep™ Mouse CD11c Positive Selection Kit II 18780 All English
Safety Data Sheet 3 EasySep™ Mouse CD11c Positive Selection Kit II 18780 All English
Safety Data Sheet 4 EasySep™ Mouse CD11c Positive Selection Kit II 18780 All English
Safety Data Sheet 1 RoboSep™ Mouse CD11c Positive Selection Kit II 18780RF All English
Safety Data Sheet 2 RoboSep™ Mouse CD11c Positive Selection Kit II 18780RF All English
Safety Data Sheet 3 RoboSep™ Mouse CD11c Positive Selection Kit II 18780RF All English
Safety Data Sheet 4 RoboSep™ Mouse CD11c Positive Selection Kit II 18780RF All English
Safety Data Sheet 1 EasySep™ Mouse CD11c Positive Selection Kit II with Spleen Dissociation Medium 18781 All English
Safety Data Sheet 2 EasySep™ Mouse CD11c Positive Selection Kit II with Spleen Dissociation Medium 18781 All English
Safety Data Sheet 3 EasySep™ Mouse CD11c Positive Selection Kit II with Spleen Dissociation Medium 18781 All English
Safety Data Sheet 1 RoboSep™ Mouse CD11c Positive Selection Kit II with Spleen Dissociation Medium 18781RF All English
Safety Data Sheet 2 RoboSep™ Mouse CD11c Positive Selection Kit II with Spleen Dissociation Medium 18781RF All English
Safety Data Sheet 3 RoboSep™ Mouse CD11c Positive Selection Kit II with Spleen Dissociation Medium 18781RF All English
数据及文献

Data

Typical EasySep™ CD11c Positive Selection Profile

Figure 1. Typical EasySep™ CD11c Positive Selection Profile

Starting with mouse splenocytes, the CD11c+ cell content of the enriched fraction is typically 86.8 ± 9.7% (gated on viable singlet cells, mean ± SD using the purple EasySep™ Magnet). In the example above, the final purities of the start and isolated fraction are 5.7% and 92.3%, respectively.

Publications (4)

Nature communications 2020 PD-L1 on dendritic cells attenuates T cell activation and regulates response to immune checkpoint blockade. Q. Peng et al.

Abstract

Immune checkpoint blockade therapies have shown clinical promise in a variety of cancers, but how tumor-infiltrating T cells are activated remains unclear. In this study, we explore the functions of PD-L1 on dendritic cells (DCs), which highly express PD-L1. We observe that PD-L1 on DC plays a critical role in limiting T cell responses. Type 1 conventional DCs are essential for PD-L1 blockade and they upregulate PD-L1 upon antigen uptake. Upregulation of PD-L1 on DC is mediated by type II interferon. While DCs are the major antigen presenting cells for cross-presenting tumor antigens to T cells, subsequent PD-L1 upregulation protects them from killing by cytotoxic T lymphocytes, yet dampens the antitumor responses. Blocking PD-L1 in established tumors promotes re-activation of tumor-infiltrating T cells for tumor control. Our study identifies a critical and dynamic role of PD-L1 on DC, which needs to be harnessed for better invigoration of antitumor immune responses.
Nature communications 2020 Senolytics prevent mt-DNA-induced inflammation and promote the survival of aged organs following transplantation. J. Iske et al.

Abstract

Older organs represent an untapped potential to close the gap between demand and supply in organ transplantation but are associated with age-specific responses to injury and increased immunogenicity, thereby aggravating transplant outcomes. Here we show that cell-free mitochondrial DNA (cf-mt-DNA) released by senescent cells accumulates with aging and augments immunogenicity. Ischemia reperfusion injury induces a systemic increase of cf-mt-DNA that promotes dendritic cell-mediated, age-specific inflammatory responses. Comparable events are observed clinically, with the levels of cf-mt-DNA elevated in older deceased organ donors, and with the isolated cf-mt-DNA capable of activating human dendritic cells. In experimental models, treatment of old donor animals with senolytics clear senescent cells and diminish cf-mt-DNA release, thereby dampening age-specific immune responses and prolonging the survival of old cardiac allografts comparable to young donor organs. Collectively, we identify accumulating cf-mt-DNA as a key factor in inflamm-aging and present senolytics as a potential approach to improve transplant outcomes and availability.
Immunity 2017 AUG Dendritic Cells but Not Macrophages Sense Tumor Mitochondrial DNA for Cross-priming through Signal Regulatory Protein α Signaling. Xu MM et al.

Abstract

Inhibition of cytosolic DNA sensing represents a strategy that tumor cells use for immune evasion, but the underlying mechanisms are unclear. Here we have shown that CD47-signal regulatory protein α (SIRPα) axis dictates the fate of ingested DNA in DCs for immune evasion. Although macrophages were more potent in uptaking tumor DNA, increase of DNA sensing by blocking the interaction of SIRPα with CD47 preferentially occurred in dendritic cells (DCs) but not in macrophages. Mechanistically, CD47 blockade enabled the activation of NADPH oxidase NOX2 in DCs, which in turn inhibited phagosomal acidification and reduced the degradation of tumor mitochondrial DNA (mtDNA) in DCs. mtDNA was recognized by cyclic-GMP-AMP synthase (cGAS) in the DC cytosol, contributing to type I interferon (IFN) production and antitumor adaptive immunity. Thus, our findings have demonstrated how tumor cells inhibit innate sensing in DCs and suggested that the CD47-SIRPα axis is critical for DC-driven antitumor immunity.
Journal of Immunology 2016 MAY LSm14A Plays a Critical Role in Antiviral Immune Responses by Regulating MITA Level in a Cell-Specific Manner. Liu T-T et al.

Abstract

Viral infection triggers induction of antiviral cytokines and effectors, which are critical mediators of innate antiviral immune response. It has been shown that the processing body-associated protein LSm14A is involved in the induction of antiviral cytokines in cell lines but in vivo evidence is lacking. By generating LSm14A-deficient mice, in this study, we show that LSm14A plays a critical and specific role in the induction of antiviral cytokines in dendritic cells (DCs) but not in macrophages and fibroblasts. Induction of antiviral cytokines triggered by the DNA viruses HSV-1 and murid herpesvirus 68 and the RNA virus vesicular stomatitis virus but not Sendai virus was impaired in Lsm14a(-/-) DCs, which is correlated to the functions of the adaptor protein MITA/STING in the antiviral signaling pathways. LSm14A deficiency specifically downregulated MITA/STING level in DCs by impairing its nuclear mRNA precursor processing and subsequently impaired antiviral innate and adaptive immune responses. Our findings reveal a nuclear mRNA precursor processing and cell-specific regulatory mechanism of antiviral immune responses.
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