Annexin V Binding Buffer

Annexin V Binding Buffer is an isotonic buffer used in conjunction with Annexin V dyes (e.g. Catalog #100-0329) and either 7-AAD (Catalog #75001) or Propidium Iodide (Catalog #75002). This buffer contains an optimal concentration of calcium that facilitates the binding of Annexin V to phosphatidylserine on the cell surface, which is essential for the detection of early-stage cell apoptosis by flow cytometry.