Annexin V Apoptosis Detection Kit with 7-AAD
Apoptosis staining and detection kit
概要
The Annexin V Apoptosis Detection Kit includes Annexin V (Catalog #100-0329 or Catalog #100-0331 or Catalog #100-0333), Annexin V Binding Buffer (Catalog #100-0334), and 7-AAD (Catalog #75001). The kit is used for the combined detection of early-stage cell apoptosis (using Annexin V) and late-stage cell apoptosis or necrosis (using both Annexin V and 7-AAD).
Annexin V belongs to the annexin family of intracellular proteins that bind to membrane phospholipids in a calcium-dependent manner. This dye has a high affinity for phosphatidylserine (PS) that is present in the inner leaflet of the plasma membrane. During early-stage cell apoptosis, PS is translocated from the inner to the outer leaflet of the cell membrane, exposing it to the external environment, where it is detected by Annexin V. This process of PS translocation occurs prior to the loss of membrane integrity. As cells progress through apoptosis and towards necrosis, the cell membrane is compromised and consequently the 7-AAD viability dye passes into the cell. Thus, cells undergoing early apoptosis stain positive for Annexin V and negative for viability dyes, while apoptotic death or necrosis is characterized by positive staining for both Annexin V and 7-AAD.
Annexin V belongs to the annexin family of intracellular proteins that bind to membrane phospholipids in a calcium-dependent manner. This dye has a high affinity for phosphatidylserine (PS) that is present in the inner leaflet of the plasma membrane. During early-stage cell apoptosis, PS is translocated from the inner to the outer leaflet of the cell membrane, exposing it to the external environment, where it is detected by Annexin V. This process of PS translocation occurs prior to the loss of membrane integrity. As cells progress through apoptosis and towards necrosis, the cell membrane is compromised and consequently the 7-AAD viability dye passes into the cell. Thus, cells undergoing early apoptosis stain positive for Annexin V and negative for viability dyes, while apoptotic death or necrosis is characterized by positive staining for both Annexin V and 7-AAD.
Components
- Annexin V (Catalog #100-0329 or Catalog #100-0331 or Catalog #100-0333),1X 100 Tests
- Annexin V Binding Buffer (Catalog #100-0334), 1 x 50 mL
- 7-AAD (Catalog #75001), 1X 200 Tests
Conjugation
APC, FITC, PE
Application
Flow Cytometry
Area of Interest
Drug Discovery and Toxicity Testing, Immunology
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | Annexin V Apoptosis Detection Kit with 7-AAD, PE | 100-0337 | All | English |
| Product Information Sheet | Annexin V Apoptosis Detection Kit with 7-AAD, FITC | 100-0338 | All | English |
| Product Information Sheet | Annexin V Apoptosis Detection Kit with 7-AAD, APC | 100-0339 | All | English |
数据及文献
Data
Figure 1. Data for Annexin V, APC
Flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with APC-conjugated Annexin V and 7-AAD (Catalog #75001).
Figure 2. Data for Annexin V, FITC
Flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with FITC-conjugated Annexin V and 7-AAD (Catalog #75001).
Figure 3. Data for Annexin V, PE
Figure showing flow cytometry analysis of C57BL/6 mouse thymocytes incubated at 37°C with 1 µM dexamethasone overnight. Cells were harvested and labeled with PE-conjugated Annexin V and 7-AAD.