NeuroFluor™ NeuO

Membrane-permeable fluorescent probe for the detection of live neurons

概要技术资料数据及文献

概要

NeuroFluor™ NeuO is a membrane-permeable fluorescent probe that selectively labels primary and pluripotent stem cell-derived neurons in live cultures (J C Er et al. Angew Chem Int Ed, 2015). Cells labeled with NeuroFluor™ NeuO can be visualized using fluorescent imaging. Labeling with this probe is non-permanent; it can be washed off, providing unlabeled, viable cells for downstream applications. For additional information, see References.

• Enables selective labeling of mouse, rat, or human neurons without fixation
• Can be used to confirm neuronal differentiation of human pluripotent stem cell-derived neural progenitor cells
• Can be used to label neurons in live culture
• Non-toxic and non-permanent
• Simple and rapid labeling protocol

• 100 µM NeuO (CAS Number: 1616355-50-0, 1668597-38-3)
• DMSO

Neural Cells, PSC-Derived, Neurons

Human, Mouse, Rat

Characterization, Phenotyping

NeuroFluor

Disease Modeling, Neuroscience, Stem Cell Biology

67-68-5

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	NeuroFluor™ NeuO	01801	All	English
Safety Data Sheet	NeuroFluor™ NeuO	01801	All	English

数据及文献

Data

Figure 1. NeuroFluor™ NeuO Selectively Labels Primary E18 Rat Neurons

(A) Primary rat E18 cortical neurons were labeled with 0.25μM NeuroFluor™ NeuO (green) and incubated for 1 hour. Image was taken after 2 hours of incubation. (B) The same culture was later fixed and stained for β-tubulin III (red). The image shows that NeuroFluor™ NeuO specifically labels β-tubulin III-positive neurons. Nuclei are counterstained with DAPI. Images were taken at 20X magnification.

Figure 2. NeuroFluor™ NeuO Selectively Labels hPSC-Derived Neurons

(A) The neuronal precursors generated from hPSC-derived (XCL-1) neural progenitor cells were cultured in STEMdiff™ Neuron Maturation Medium. After 18 days of culture, hPSC-derived neurons were labeled with NeuroFluor™ NeuO (green). Nuclei are counterstained with DAPI. (B) The same culture was later fixed and stained with β-tubulin III (red). The image shows that NeuroFluor™ NeuO specifically labels β-tubulin III-positive neurons. Images were taken at 20X magnification.

Publications (1)

Angewandte Chemie - International Edition 2015 FEB Neuo: A fluorescent chemical probe for live neuron labeling Er JC et al.

Abstract

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Abstract

To address existing limitations in live neuron imaging, we have developed NeuO, a novel cell-permeable fluorescent probe with an unprecedented ability to label and image live neurons selectively over other cells in the brain. NeuO enables robust live neuron imaging and isolation in vivo and in vitro across species; its versatility and ease of use sets the basis for its development in a myriad of neuronal targeting applications.

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