GloCell™ Fixable Viability Dye Violet 450
Amine-labeling fluorescent dye for live/dead staining of mammalian cells
概要
GloCell™ Fixable Viability Dye Violet 450 is a fluorescent cell viability dye for staining live/dead mammalian cells in applications such as flow cytometry. The dye irreversibly binds intracellular and cell surface amine groups and can be utilized prior to fixation, permeabilization, or cryopreservation. Cells with compromised plasma membranes become permeable to the GloCell™ dye, resulting in a greater fluorescence compared to live cells in a cell viability assay.
GloCell™ Fixable Viability Dye Violet 450 is excited by the violet laser at 405 nm and it has a fluorescence emission maximum of 450 nm that can be detected using the 440/40 or 440/50 band pass filter used to detect Pacific Blue™.
GloCell™ Fixable Viability Dye Violet 450 is excited by the violet laser at 405 nm and it has a fluorescence emission maximum of 450 nm that can be detected using the 440/40 or 440/50 band pass filter used to detect Pacific Blue™.
Species
Human, Mouse, Rat, Non-Human Primate, Other
Application
Flow Cytometry, Immunofluorescence
Brand
GloCell
Area of Interest
Angiogenic Cell Research, Antibody Development, Cancer Research, Cell Line Development, Cell Therapy, Chimerism, Disease Modeling, Drug Discovery and Toxicity Testing, Endothelial Cell Biology, Epithelial Cell Biology, HIV, HLA, Hybridoma Generation, Immunology, Infectious Diseases, Metabolism, Mitosis, Neuroscience, Organoids, Respiratory Research, Stem Cell Biology, Transplantation Research
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | GloCell™ Fixable Viability Dye Violet 450 | 75009, 75009.1 | All | English |
| Safety Data Sheet | GloCell™ Fixable Viability Dye Violet 450 | 75009, 75009.1 | All | English |
数据及文献
Data
Figure 1.
(A) Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with GloCell™ Fixable Viability Dye Violet 450. (B) Flow cytometry analysis of human multiple myeloma bone marrow mononuclear cells (BMMCs) labeled with GloCell™ Fixable Viability Dye Violet 450.
Figure 2.
Excitation/Emission spectra for GloCell™ Fixable Viability Dye Violet 450. Excitation max: 403 nm, Emission Max: 454 nm