Phalloidin iFluor™ Conjugates

Fluorescent dye conjugates for detection of F-actin in cell culture experiments
概要

Phalloidin is a heptapeptide derived from the poisonous mushroom Amanita phalloides. Phalloidin binds with a high specificity to F-actin filaments in cells, preventing filament depolymerization. Fluorescently labeled phalloidin can be used in labeling, identification, and quantitation of F-actin in formaldehyde-fixed and permeabilized cells. Fluorescently labeled phalloidin has lower non-specific binding compared to actin antibodies across most species of plants and animals, which provides low background and high contrast during imaging.

Cell Type
PSC衍生神经细胞, 多能干细胞, 神经元
Application
表征
Area of Interest
干细胞生物学, 癌症研究, 神经科学
CAS Number
67-68-5


技术资料

Scientific Resources


数据及文献

Product Applications

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Data and Publications

数据

Image of H9 embryonic stem cells stained with Phalloidin-iFluor™ 647.

Figure 1. Phalloidin-iFluor™ Can Be Used to Stain Embryonic Stem Cells

H9 embryonic stem cells were plated in mTeSR™ Plus (Catalog #100-0276) on Corning® Matrigel®-coated 35 mm glass-bottom dishes for 24 hours, then fixed with 4% paraformaldehyde and stained with Phalloidin-iFluor™ 647 (red), and counterstained with Hoechst 33342 (blue).

Image of forebrain-type neurons stained with Phalloidin-iFluor™ 647.

Figure 2. Phalloidin-iFluor™ Can Be Used to Stain Forebrain-Type Neurons

WLS-1C cell-derived neural progenitor cells were differentiated to forebrain-type neurons using STEMdiff™ Forebrain Neuron Differentiation (Catalog #08600) and STEMdiff™ Forebrain Neuron Maturation Kits (Catalog #08605). Neurons were cultured on poly-L-ornithine/laminin-coated 35 mm glass-bottom dishes for 3 weeks, then fixed with 4% paraformaldehyde and stained with Phalloidin-iFluor™ 647 (red), and counterstained with Hoechst 33342 (blue).


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