MesenCult™-hPL Medium Kit

For the culture of human mesenchymal stem cells
概要
MesenCult™-hPL Medium has been optimized for the culture and expansion of human mesenchymal stromal cells (MSCs; also known as mesencyhymal stem cells) as well as for the detection and enumeration of colony-forming unit fibroblasts (CFU-F). MesenCult™-hPL 10X Supplement is an alternative growth supplement to fetal bovine serum (FBS) that contains purified human platelet lysate (hPL). Complete MesenCult™-hPL Medium (Basal Medium + 10X Supplement) does not require addition of growth factors, lipids, or attachment substrate. It is more defined and provides better performance than FBS-containing media, and is also a more affordable option than defined, serum-free media.

Certain products are only available in select territories. Please contact your local Sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.
Advantages
• Superior performance in terms of CFU-F numbers and long-term expansion compared to FBS-containing medium
• Cheaper medium compared to serum-free, defined media
• More consistent and sustainable raw materials (less batch-to-batch variability)
• Complete supplement; addition of growth factor is unnecessary
• Attachment substrate is not required; saves time and money
Components
  • MesenCult™-hPL Basal Medium, 450 mL
  • MesenCult™-hPL 10X Supplement, 50 mL
Subtype
Specialized Media
Cell Type
Mesenchymal Stem and Progenitor Cells
Species
Human
Application
Cell Culture, Colony Assay, Expansion
Brand
MesenCult
Area of Interest
Stem Cell Biology
技术资料
Document Type 产品名称 Catalog # Lot # 语言
Product Information Sheet 1 MesenCult™-hPL Medium Kit 05439 Supplement 17E78846 or higher English
Product Information Sheet 2 MesenCult™-hPL Medium Kit 05439 Supplement 17E78846 or higher English
Safety Data Sheet 1 MesenCult™-hPL Medium Kit 05439 All English
Safety Data Sheet 2 MesenCult™-hPL Medium Kit 05439 All English
数据及文献

Data

Figure 1. Bone marrow (BM)-derived MSCs Cultured in MesenCult™-hPL Medium Expand Faster than MSCs Cultured in Serum (FBS)-containing medium

Figure 2. Human BM-derived MSCs Cultured in MesenCult™-hPL Medium Display Multi-Lineage Differentiation Potential

(A) Human BM-derived MSCs cultured in MesenCult™-HPL Medium differentiate into (B) adipocytes (Oil Red O staining); and (C) chondrocytes (Alcian Blue staining); and (D) osteoblasts (alkaline phosphatase and von Kossa staining).

Figure 3. Flow Cytometric Analysis of Cultured-Expanded MSCs in MesenCult™-hPL Medium

BM-derived MSCs were cultured and expanded in MesenCult™-hPL Medium. MSCs were stained for the mesenchymal surface markers CD73, CD90 and CD105, and the hematopoietic marker, CD45. MSCs express high levels of CD73, CD90 and CD105 while lacking expression of CD45.

Publications (1)

Cellular {\&} molecular immunology 2020 oct Improved therapeutic consistency and efficacy of mesenchymal stem cells expanded with chemically defined medium for systemic lupus erythematosus. J. Xu et al.
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