mTeSR™1

cGMP, feeder-free maintenance medium for human ES and iPS cells
概要
mTeSR™1, now manufactured under cGMP, is the most widely published feeder-free cell culture medium for human embryonic stem cells (ES cells) and induced pluripotent stem cells (iPS cells), with established protocols for applications ranging from derivation to differentiation. It has been used to successfully maintain thousands of ES and iPS cell lines in over 50 countries, and has supported top pluripotent stem cell publications and researchers. mTeSR™1 is a highly specialized, serum-free and complete cell culture medium. With pre-screened raw materials that ensure batch-to-batch consistency and robust feeder-free protocols for ES and iPS cell culture, mTeSR™1 provides more consistent cultures with homogeneous, undifferentiated phenotypes.

mTeSR™1 is manufactured under relevant cGMPs, ensuring the highest quality and consistency for reproducible results.

To request a Letter of Authorization (LOA) for mTeSR’s Drug Master File, click here.                                
Components
  • mTeSR™1 Complete Kit (Catalog #85850)
    • mTeSR™1 Basal Medium, 400 mL
    • mTeSR™1 5X Supplement, 100 mL
  • mTeSR™1 Complete Kit, 1 L (Catalog #85857)
    • mTeSR™1 Basal Medium, 800 mL
    • mTeSR™1 5X Supplement, 100 mL, 2 Bottles
  • mTeSR™1 Complete Kit, 10 Pack (Catalog #85870)
    • mTeSR™1 Basal Medium, 400 mL, 10 Bottles
    • mTeSR™1 5X Supplement, 100 mL, 10 Bottles
  • mTeSR™1 Complete Kit, 25 Pack (Catalog #85875)
    • mTeSR™1 Basal Medium, 400 mL, 25 Bottles
    • mTeSR™1 5X Supplement, 100 mL, 25 Bottles
Subtype
Specialized Media
Cell Type
Pluripotent Stem Cells
Species
Human
Application
Cell Culture, Expansion, Maintenance
Brand
TeSR
Area of Interest
Stem Cell Biology
Formulation
Serum-Free
数据及文献

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages
Human Pluripotent Stem Cell Research
Reprogramming
 
Maintenance
 
Differentiation
PSC Quality Control
View All

Data and Publications

Data

 

Figure 1. Normal hES and hiPS Cell Morphology is Observed in cGMP mTeSR™1 Cultures

Undifferentiated (A) H1 human embryonic stem (hES) and (B) WLS-1C human induced pluripotent stem (hiPS) cells cultured on Corning® Matrigel® Matrix in cGMP mTeSR™1 retain the prominent nucleoli and high nuclear-to-cytoplasmic ratio characteristic of this cell type after 10 passages. Densely packed cells and multi-layering are prominent when cells are ready to be passaged.

Figure 2. High Expansion Rates are Observed in cGMP mTeSR™1 Cultures

Graph shows the average fold expansion per passage +/- SEM obtained for hES (H1 and H9) and hiPS (WLS-1C) cells cultured in cGMP mTeSR­™1 (red) or non-cGMP mTeSR™1 (gray) on Corning® Matrigel® Matrix over 10 passages. Expansion was determined by enumerating the cell aggregates obtained at harvest and dividing by the number of cell aggregates seeded. Note that this data is representative of cultures passaged after 6-7 days in culture, lower expansion should be expected if using shorter culture times.

Figure 3. Cells Cultured in cGMP mTeSR™1 Medium Express Undifferentiated Cell Markers

Histogram analysis for hES (H1 and H9) and hiPS (WLS-1C) cells characterized using FACS for undifferentiated cell markers, OCT4 (OCT3) (Catalog #60093) and TRA-1-60 (Catalog #60064), after 8 - 10 passages in cGMP mTeSR™1 (filled = sample, blank = isotype control).

Figure 4. hPSCs Maintained in cGMP mTeSR™1 Display a Normal Karyotype

Karyograms of (A) H1 hES and (B) WLS-1C hiPS cells cultured in cGMP mTeSR™1 for 11 passages shows that a normal karyotype is retained.


Legal Statement:

This product was developed under license to intellectual property owned by WiCell™ Research Institute. This product is sold for research use only (whether the buyer is an academic or for-profit entity) under a non-transferable, limited-use license. Purchase of this product does not include the right to sell, use or otherwise transfer this product for commercial purposes (i.e., any activity undertaken for consideration, such as use of this product for manufacturing, or resale of this product or any materials made using this product, or use of this product or any materials made using this product to provide services) or clinical use (i.e., administration of this product or any material using this product to humans) or the right to implant any material made using this product into an animal by, or in collaboration with, a for-profit entity, for purposes other than basic pre-clinical research applications (including without limitation teratoma assays) to validate the function of the cells. Purchasers who do not agree to the terms and conditions set forth above should return the product in acceptable conditions to the seller for a refund.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL。


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