ClonaCell™-CHO ACF Supplement

Animal component-free, serum-free, glutamine-free 40X supplement for cloning during CHO cell line development
概要
ClonaCell™-CHO ACF Supplement is a serum-free and animal component-free supplement that is added to protein-free media in order to increase cloning efficiency and promote robust growth of CHO cells. The 40X supplement can be added to a variety of protein-free liquid or semi-solid cell culture media to increase the growth and single-cell cloning efficiency of suspension-adapted CHO cells. The supplement contains recombinant proteins and phenol red, and does not contain animal components, serum, hydrolysates, L-glutamine, or selection agents.
Advantages
• Significantly increases single-cell cloning efficiency of CHO cells in protein-free liquid or semi-solid media
• Compatible with a wide variety of commercially available liquid or semi-solid protein-free media
• Allows the user to maintain existing upstream and downstream cell culture processes
Contains
• Recombinant proteins, phenol red, and chemically defined components
Subtype
Supplements
Cell Type
CHO Cells
Species
Other
Application
Cell Culture
Brand
ClonaCell
Area of Interest
Antibody Development, Cell Line Development, Drug Discovery and Toxicity Testing
Formulation
Animal Component-Free, Serum-Free
技术资料
Document Type 产品名称 Catalog # Lot # 语言
Product Information Sheet ClonaCell™-CHO ACF Supplement 03820 All English
Safety Data Sheet ClonaCell™-CHO ACF Supplement 03820 All English
数据及文献

Data

Cloning efficiencies of CHO-S and CHO-K1 cells in protein-free media from different commercial suppliers with and without addition of ClonaCell™-CHO ACF Supplement

Figure 1. Cloning efficiencies of CHO-S and CHO-K1 cells in protein-free media from different commercial suppliers with and without addition of ClonaCell™-CHO ACF Supplement

The bar graphs illustrate the cloning efficiency of untransfected CHO-S (A) and CHO-K1 (B) cells, defined as the percentage of wells in 96-well plates that contain greater than 100 cells after 14 days of incubation. Individual wells of a 96-well plate were seeded with an average cell density of one cell per well in 200 µL cell culture medium and incubated at 37°C and 5% CO 2. All conditions contained 6 - 8 mM L-glutamine. Cloning efficiencies in various protein-free cell culture media with (blue) or without (grey) addition of ClonaCell™-CHO ACF Supplement or in DMEM + 10% FBS (orange bar) are shown. Error bars represent the standard deviation based on triplicate data for each condition, except Medium 2 (both with and without ClonaCell™-CHO ACF Supplement), for which the average of duplicate data is shown. Medium 1 = ClonaCell™-CHO CD Liquid Medium, Medium 2 = Gibco® CD CHO Medium, Medium 3 = Gibco® CD OptiCHO™ Medium, Medium 4 = Lonza PowerCHO™2.

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