CD34+ Progenitor Reprogramming Kit
• Improved reprogramming efficiency and higher frequency of iPS cell colonies, compared to traditional hES cell medium
• Rapid emergence of large colonies with high quality iPS cell-like morphology facilitates identification and subcloning
• Seamlessly integrates with TeSR™ and STEMdiff™ products for downstream maintenance and differentiation of iPS cell lines
- SepMate™-50 (Catalog #15440)
- Lymphoprep™ (Catalog #07801)
- Complete Kit for Human Whole Blood CD34+ Cells (Catalog #15086)
- RosetteSep™ Human Hematopoietic Progenitor Cell Enrichment Cocktail, 3 x 2 mL
- EasySep™ Human CD34 Positive Selection Cocktail, 0.4 mL
- EasySep™ Dextran RapidSpheres™ 50100, 1 mL
- StemSpan™ SFEM II (Catalog #09605)
- StemSpan™ CD34+ Expansion Supplement (Catalog #02691)
- ReproTeSR™ (Catalog #05926)
- TeSR™-E7™/ReproTeSR™ Basal Medium
- ReproTeSR™ 25X Supplement
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Manual | CD34+ Progenitor Reprogramming Kit | 05925 | All | English |
| Safety Data Sheet 1 | CD34+ Progenitor Reprogramming Kit | 05925 | All | English |
| Safety Data Sheet 2 | CD34+ Progenitor Reprogramming Kit | 05925 | All | English |
| Safety Data Sheet 3 | CD34+ Progenitor Reprogramming Kit | 05925 | All | English |
| Safety Data Sheet 4 | CD34+ Progenitor Reprogramming Kit | 05925 | All | English |
Data
Figure 1. Typical Performance of the EasySep™ Complete Kit for Human Whole Blood CD34+ Cells
(A) In whole PB, the proportion of CD34+/CD45+ cells is typically very low (<1%). (B) In the RosetteSep™ enrichment step, unwanted cells are removed during density gradient centrifugation. This step enriches the population of CD34+/CD45+ cells (19.8%) in this example). (C) After positive selection of CD34+ cells using EasySep™, a highly pure population of CD34+/CD45+ cells (96.2% in this example) can be obtained. Values are reported as a percentage of viable CD45+ cells.
Figure 2. StemSpan™ SFEM II and CD34+ Expansion Supplement Support Expansion of CD34+ Cells
TNC: Total Nucleated Cell
Figure 3. Schematic of ReproTeSR™ Reprogramming Timeline
ReproTeSR™ is used during the entire induction phase of reprogramming (day 3 to 21). On days 3 and 5, ReproTeSR™ is added to StemSpan™ growth media (in a fed-batch manner) to facilitate attachment of transfected cells. Attached cells are further cultured in ReproTeSR™ with daily full media changes until putative iPS cell colonies emerge (days 21-28). iPS cell colonies can then be isolated and propagated in TeSR™ media. (mTeSR™1, TeSR™2, TeSR™-E8™).
Figure 4. Blood Cell Reprogramming Efficiencies Are Higher in ReproTeSR™ Medium Compared to in hESC Medium
Efficiency of reprogramming (A) erythroid cells, or (B) CD34+ cells using episomal reprogramming vectors is higher in ReproTeSR™ medium compared to in KOSR-containing hESC medium. Data shown are mean +/- SEM, erythroid cells n=4, CD34+ cells n=5.
Figure 5. ReproTeSR™ Generates iPS Cell Colonies With Superior Colony Morphology
Representative images of iPS cell colonies generated from isolated CD34+ progenitor cells using (A) ReproTeSR™ and (B) hESC) medium. iPS cell colonies produced using ReproTeSR™ exhibit more defined borders, compact morphology and reduced differentiation compared with hESC medium. 200X magnification.
Figure 6. Reprogramming Efficiency of CD34+ and Erythroid Progenitor Cells With ReproTeSR™
Average values in bold (range).