STEMdiff™ Sensory Neuron Maturation Kit
Maturation kit for generation of functional sensory neurons from human iPS cell-derived sensory neuronal precursor cells
⦁ Mature and maintain sensory neurons seamlessly following STEMdiff™ Sensory Neuron Differentiation Kit
⦁ Streamline PSC-derived sensory neuron generation with a simple, easy-to-use media format
⦁ Obtain physiologically relevant results with integrated BrainPhys™ media supporting neuronal activity and maturation
- BrainPhys™ Neuronal Medium, 100 mL (Catalog #05797)
- STEMdiff™ Sensory Neuron Maturation Supplement, 25 mL
Scientific Resources
Product Documentation
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | STEMdiff™ Sensory Neuron Maturation Kit | 100-0684 | All | English |
| Safety Data Sheet 1 | STEMdiff™ Sensory Neuron Maturation Kit | 100-0684 | 全部 | English |
| Safety Data Sheet 2 | STEMdiff™ Sensory Neuron Maturation Kit | 100-0684 | 全部 | English |
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
数据
Figure 1. Schematic for the STEMdiff™ Sensory Neuron Culture System Protocol
Sensory neurons can be generated in 7 days from sensory neuron precursors after 6 days in STEMdiff™ Sensory Neuron Differentiation Medium. For the differentiation of sensory neuron precursors from hPSC-derived neural crest cells, see the PIS.
Figure 2. STEMdiff™ Sensory Neuron Kits Promote Differentiation Across Multiple Embryonic Stem and Induced Pluripotent Stem Cell Lines
NCCs generated from hPSCs in mTeSR™ Plus using the STEMdiff™ Neural Crest Differentiation Kit were differentiated and matured to sensory neurons using the STEMdiff™ Sensory Neuron Differentiation and Maturation Kits. (A) Sensory neurons were generated after hPSC-derived NCCs were cultured with the STEMdiff™ Sensory Neuron Differentiation Kit for 6 days and then the STEMdiff™ Sensory Neuron Maturation Kit for 6 days. The resulting cultures contain a population of cells expressing sensory neuron markers peripherin (green) and BRN3A (red) along with (B) neuronal marker class III β-tubulin (TUJ1, red). (C) Midbrain neuron controls generated with STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits do not have detectable peripherin (green) or BRN3A (red) expression, although they express (D) neuronal marker class III β-tubulin (TUJ1, red). Nuclei are labeled with DAPI (blue). Human ES and iPS cell lines were maintained in either mTeSR™1, TeSR™-E8™, or mTeSR™ Plus and differentiated with STEMdiff™ Neural Crest Differentiation Kit, followed by STEMdiff™ Sensory Neuron Differentiation and Maturation Kits. The percentage expression of (E) BRN3A+ and (F) TUJ1+ cells in the resulting cultures was quantified. This differentiation generated BRN3A+ sensory neurons (25.3% ± 6.9%, mean ± SEM; n=7 cell lines, 3 - 23 replicates per condition) that expressed neuronal marker class III β-tubulin (TUJ1; 90.3% ± 4.1%, mean ± SEM; n=4 cell lines, 3 - 12 replicates per condition). Numbers are % positive over total DAPI in a tiled image. NCCs = neural crest cells; hPSCs = human pluripotent stem cells; ES = embryonic stem; iPS = induced pluripotent stem