StemSpan™-ACF Erythroid Expansion Medium

Animal component-free medium for culture and expansion of human erythroid cells

概要技术资料数据及文献

概要

StemSpan™-ACF Erythroid Expansion Medium (ACF-E) is an animal component-free (ACF) medium for the in vitro culture and expansion of human hematopoietic cells. When supplemented with StemSpan™ Erythroid Expansion Supplement (100X; Catalog #02692), this medium supports the expansion and erythroid differentiation of human hematopoietic progenitor cells into CD71+ and Glycophorin A+ erythroblasts in ACF culture conditions. This medium only contains recombinant proteins and synthetic components; it does not contain serum or other human- or animal-derived components.

⦁ Animal component-free conditions specifically designed for expansion and differentiation of erythroid progenitor cells
⦁ Optimized for use with StemSpan™ Erythroid Expansion Supplement
⦁ Produces thousands of CD71+GlyA+ erythroid cells per input CD34+ cell

This product contains only recombinant proteins and synthetic components

Specialized Media

Erythroid Cells, Hematopoietic Stem and Progenitor Cells

Human

Cell Culture, Differentiation, Expansion

StemSpan

Stem Cell Biology, Transplantation Research

Animal Component-Free, Serum-Free

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	StemSpan™-ACF Erythroid Expansion Medium	09860	All	English
Safety Data Sheet	StemSpan™-ACF Erythroid Expansion Medium	09860	All	English

数据及文献

Data

Figure 1. Production of Erythroid Cells from Human CB-Derived CD34+ Cells Cultured in StemSpan™ Media Containing StemSpan™ Erythroid Expansion Supplement

(A) Average numbers of erythroid cells generated after culturing purified CD34+ CB cells (n = 5) for 14 days in StemSpan™ SFEM (black bars), SFEM II (grey bars), or StemSpan™-ACF Erythroid Expansion Medium (ACF-E, orange bars) media containing StemSpan™ Erythroid Expansion Supplement (Catalog #02692). Shown are the number of erythroid cells that express CD71 and/or Glycophorin A (GlyA) per input CD34+ cell. (B) The percentages of the different erythroid cell subsets generated in these cultures are shown, including CD71+GlyA+ erythroblasts, immature CD71+GlyA- erythroid progenitor cells and pro-erythroblasts, and CD71-/lowGlyA+ normoblasts. All three media supported the generation of thousands of erythroid cells per CB-derived CD34+ cell plated.

Table 1. Production of Erythroid Cells from Human CB-Derived CD34+ Cells Cultured in StemSpan™ Media Containing StemSpan™ Erythroid Expansion Supplement

Yields and percentages of erythroid cells produced after culturing purified CD34+ CB cells (n = 5) for 14 days in StemSpan™ SFEM, SFEM II or ACF-E media containing Erythroid Expansion Supplement. Erythroid cells were identified by flow cytometry after staining with antibodies against CD71 and GlyA. The % erythroid cells represent the percentage of cells that express CD71 and/or GlyA.

*CI: Confidence Interval.