STEMdiff™ Atrial Cardiomyocyte Differentiation Kit
Culture medium kit for differentiation of human PSCs to atrial cardiomyocytes
概要
STEMdiff™ Atrial Cardiomyocyte Differentiation Kit is optimized for the differentiation and maintenance of human embryonic stem and induced pluripotent stem cells into atrial cardiomyocytes (cardiac troponin T-positive; cTnT+). This kit can be used to generate atrial cardiomyocytes derived from a clump culture of human pluripotent stem cells (hPSCs) maintained in mTeSR™1 (Catalog #85850), mTeSR™ Plus (Catalog #100-0276), or TeSR™-E8™ (Catalog #05990). With greater than 80% yield purity, an average of 1 x 10^6 cTnT+ cells can be harvested from a single well of a 12-well plate.
STEMdiff™ Cardiomyocyte Maintenance Kit (Catalog #05020) comprises the maintenance basal medium and supplement and allows for long-term maintenance of hPSC-derived atrial cardiomyocytes for one month or longer. These cardiomyocytes can be used in various downstream applications and analyses.
STEMdiff™ Cardiomyocyte Maintenance Kit (Catalog #05020) comprises the maintenance basal medium and supplement and allows for long-term maintenance of hPSC-derived atrial cardiomyocytes for one month or longer. These cardiomyocytes can be used in various downstream applications and analyses.
Advantages
⦁ Simple monolayer protocol, compatible with STEMdiff™ cardiomyocyte workflow
⦁ Establishment and efficient generation of up to 50 million atrial cardiomyocytes per kit
⦁ Cardiomyocytes generated with this kit express high levels of atrial-specific genes and demonstrate dominant atrial-like phenotypes including faster beat rate and decreased field potential duration when compared to those generated using the STEMdiff™ Cardiomyocyte Differentiation Kit
⦁ Maximum reproducibility with minimal variability across multiple hPSC lines
⦁ Establishment and efficient generation of up to 50 million atrial cardiomyocytes per kit
⦁ Cardiomyocytes generated with this kit express high levels of atrial-specific genes and demonstrate dominant atrial-like phenotypes including faster beat rate and decreased field potential duration when compared to those generated using the STEMdiff™ Cardiomyocyte Differentiation Kit
⦁ Maximum reproducibility with minimal variability across multiple hPSC lines
Components
- STEMdiff™ Cardiomyocyte Differentiation Basal Medium, 380 mL
- STEMdiff™ Atrial Cardiomyocyte Differentiation Supplement A (10X), 10 mL
- STEMdiff™ Atrial Cardiomyocyte Differentiation Supplement B (10X), 10 mL
- STEMdiff™ Atrial Cardiomyocyte Differentiation Supplement C (10X), 20 mL
- STEMdiff™ Cardiomyocyte Maintenance Basal Medium, 490 mL
- STEMdiff™ Atrial Cardiomyocyte Maintenance Supplement (50X), 10 mL
Subtype
Specialized Media
Cell Type
Cardiomyocytes, PSC-Derived
Species
Human
Application
Cell Culture, Differentiation
Brand
STEMdiff
Area of Interest
Cell Line Development, Disease Modeling, Drug Discovery and Toxicity Testing, Stem Cell Biology
Formulation
Serum-Free
技术资料
| Document Type | 产品名称 | Catalog # | Lot # | 语言 |
|---|---|---|---|---|
| Product Information Sheet | STEMdiff™ Atrial Cardiomyocyte Differentiation Kit | 100-0215 | All | English |
| Special Protocol | STEMdiff™ Atrial Cardiomyocyte Differentiation Kit | 100-0215 | All | English |
| Safety Data Sheet 1 | STEMdiff™ Atrial Cardiomyocyte Differentiation Kit | 100-0215 | All | English |
| Safety Data Sheet 2 | STEMdiff™ Atrial Cardiomyocyte Differentiation Kit | 100-0215 | All | English |
| Safety Data Sheet 3 | STEMdiff™ Atrial Cardiomyocyte Differentiation Kit | 100-0215 | All | English |
| Safety Data Sheet 4 | STEMdiff™ Atrial Cardiomyocyte Differentiation Kit | 100-0215 | All | English |
| Safety Data Sheet 5 | STEMdiff™ Atrial Cardiomyocyte Differentiation Kit | 100-0215 | All | English |
| Safety Data Sheet 6 | STEMdiff™ Atrial Cardiomyocyte Differentiation Kit | 100-0215 | All | English |
数据及文献