Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136

(C3H x BALB/c) F1 hybrid monoclonal IgG2a antibody against mouse NK1.1 (CD161)
概要
The PK136 antibody reacts with murine NK1.1 (CD161), an ~80 kDa homodimeric type 2 transmembrane glycoprotein and C-type lectin receptor expressed on NK cells, NK-T cells, and rare subsets of T cells and monocytes in select strains of mice. NK1.1 is encoded by the Klrb1b and Klrb1c genes, which specify CD161b and CD161c polypeptides, respectively. CD161b is expressed only by Swiss NIH and SJL mice, and CD161c by certain strains such as C57BL, FVB/N, and NZB (but not A, AKR, BALB/c, CBA/J, C3H, C57BR, C58, DBA/1, DBA/2, NOD, SJL, or 129). NK1.1 has functional roles in modulating several processes, including the activation and proliferation of NK cells, induction of interferon-γ production, and release of cytotoxic granules. Its expression on the cell surface is specifically upregulated by IL-12. For detection of NK cells in NK1.1- strains, Anti-Mouse CD49b Antibody, Clone DX5 (Catalog #60020) is recommended. DX5 recognizes CD49b (integrin α2), another commonly used NK cell marker.
Subtype
Primary Antibodies
Target Antigen
NK1.1 (CD161)
Alternative Names
CD161b, CD161c, Klrb1b, Klrb1c, Ly-55, Ly-59, NK-1.1, NKRP1, NKRP1a, NKRP1b
Reactive Species
Mouse
Conjugation
Biotin, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
NK Cells
Species
Mouse
Application
Cell Isolation, CyTOF, Depletion, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
Area of Interest
Immunology
Clone
PK136
Gene ID
17059
Isotype
IgG2a, kappa
技术资料
Document Type 产品名称 Catalog # Lot # 语言
Product Information Sheet Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Alexa Fluor® 488 60103AD, 60103AD.1 All English
Product Information Sheet Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, APC 60103AZ, 60103AZ.1 All English
Product Information Sheet Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Biotin 60103BT, 100-0462, 100-0463 All English
Product Information Sheet Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, FITC 60103FI, 100-0464, 100-0465 All English
Product Information Sheet Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, PerCP-Cy5.5 60103PS, 60103PS.1 All English
Product Information Sheet Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Pacific Blue™ 60103PB, 60103PB.1 All English
Product Information Sheet Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136 100-0459 All English
Product Information Sheet Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, PE 100-0460, 100-0461 All English
Safety Data Sheet Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Alexa Fluor® 488 60103AD, 60103AD.1 All English
Safety Data Sheet Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, APC 60103AZ, 60103AZ.1 All English
Safety Data Sheet Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Biotin 60103BT, 100-0462, 100-0463 All English
Safety Data Sheet Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, FITC 60103FI, 100-0464, 100-0465 All English
Safety Data Sheet Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, PerCP-Cy5.5 60103PS, 60103PS.1 All English
Safety Data Sheet Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136 100-0459 All English
Safety Data Sheet Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, PE 100-0460, 100-0461 All English
数据及文献

Data

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, followed by a rat anti-mouse IgG2a antibody, PE and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC173 (Catalog #60071), followed by a rat anti-mouse IgG2a antibody, PE and Anti-Mouse CD45 Antibody, Clone 30-F11, APC.

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Alexa Fluor® 488 and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, Alexa Fluor® 488 (Catalog #60071AD) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, APC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC173, APC (Catalog #60071AZ) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Biotin, followed by streptavidin (SAV) APC and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC173, Biotin (Catalog #60071BT), followed by SAV APC and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, FITC and Anti-Mouse CD45 Antibody, Clone 30-F11, PE (Catalog #60030PE). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC173, FITC (Catalog #60071FI) and Anti-Mouse CD45 Antibody, Clone 30-F11, PE.

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, PE and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173, PE (Catalog #60071PE) and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.

Data for PerCP-Cy55-Conjugated

Figure 7. Data for PerCP-Cy55-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, PerCP-Cy5.5 and Anti-Mouse CD45 Antibody, Clone 30-F11, APC (Catalog #60030AZ).

Figure 8. Data for PB-Conjugated

(A) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with Anti-Mouse NK1.1 (CD161) Antibody, Clone PK136, Pacific Blue™ and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC (Catalog #60030FI). (B) Flow cytometry analysis of C57BL/6 mouse splenocytes (gated on lymphocytes) labeled with a mouse IgG2a, kappa Pacific Blue™ isotype control antibody and Anti-Mouse CD45 Antibody, Clone 30-F11, FITC.

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