Anti-Mouse MHC Class II (I-A/I-E), Clone M5/114.15.2

Rat monoclonal IgG2b antibody against mouse MHC Class II (I-A/I-E)
概要
The M5/114.15.2 (M5/114) antibody reacts with an extracellular epitope on the mouse major histocompatibility complex class II receptor (MHC-II). MHC-II is a heterodimeric transmembrane glycoprotein comprising an α subunit associated non-covalently with a β subunit. In mice, MHC-II complexes are designated I-A and I-E and comprise Aα/Aβ and Eα/Eβ heterodimers, respectively. Together with the CD3/T cell receptor (TCR) complex and CD4 molecules, MHC-II mediates a critical function in presenting peptides generated from hydrolysis of exogenous antigens by antigen-presenting cells to CD4+ T (helper) cells, thereby either suppressing or inducing an immune response to the peptides. The M5/114.15.2 antibody recognizes a polymorphic determinant shared by the I-Ab, I-Ad, I-Aq, I-Ed, and I-Ek (but not I-Af, I-Ak, or I-As) MHC class II alloantigens expressed on B cells, dendritic cells, macrophages, monocytes, and activated T cells of mice carrying the H-2b, d, p, q, r, and u haplotypes (but not haplotypes H-2f or H-2s). The M5/114 antibody reportedly inhibits I-A-restricted responses by T cells of the H-2b, d, q, and u (but not H-2f, k, or s) haplotypes. It does not react with cells from NOD (H-2g7) mice.
Subtype
Primary Antibodies
Target Antigen
MHC Class II (I-A/I-E)
Alternative Names
IA, I-A, IA/IE, I-A/E, I-E, MHC class II, MHC-II
Reactive Species
Mouse
Conjugation
APC, FITC, PE, Unconjugated
Host Species
Rat
Cell Type
B Cells, Macrophages, Monocytes, T Cells
Application
CyTOF, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western Blotting
Area of Interest
Immunology
Clone
M5/114.15.2
Gene ID
14961/14969
Isotype
IgG2b, kappa
数据及文献

Data

Figure showing flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, APC or a rat IgG2b, kappa APC isotype control antibody.

Figure 1. Data for Anti-Mouse MHC II Antibody, Clone M5/114.15.2, APC

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, APC (filled histogram) or a rat IgG2b, kappa APC isotype control antibody (solid line histogram).

Figure showing flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, FITC or a rat IgG2b, kappa FITC isotype control antibody.

Figure 2. Data for Anti-Mouse MHC Class II Antibody, Clone M5/114.15.2, FITC

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, FITC (filled histogram) or a rat IgG2b, kappa FITC isotype control antibody (solid line histogram).

Figure showing flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, PE or a rat IgG2b, kappa PE isotype control antibody.

Figure 3. Data for Anti-Mouse MHC Class II Antibody, Clone M5/114.15.2, PE

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, PE (filled histogram) or a rat IgG2b, kappa PE isotype control antibody (solid line histogram).

Figure showing flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, followed by a mouse anti-rat IgG2b antibody, PE or a rat IgG2b, kappa isotype control antibody followed by a mouse anti-rat IgG2b antibody, PE.

Figure 4. Data for Anti-Mouse MHC Class II Antibody, Clone M5/114.15.2,Unconjugated

Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse MHC II Antibody, Clone M5/114.15.2, followed by a mouse anti-rat IgG2b antibody, PE (filled histogram) or a rat IgG2b, kappa isotype control antibody followed by a mouse anti-rat IgG2b antibody, PE (solid line histogram).

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