Anti-Mouse CD138 (Syndecan-1) Antibody, Clone 281-2

Rat monoclonal IgG2a antibody against mouse CD138 (syndecan-1)
概要
The 281-2 antibody reacts with an extracellular epitope on the core protein of mouse CD138 (Syndecan-1), a ~60 - 100 kDa type 1 transmembrane glycoprotein expressed on the surface of pre-B cells, immature B cells, and normal and malignant plasma cells (but not mature circulating B cells). CD138 is also expressed on non-hematopoietic cells such as embryonic mesenchymal cells, endothelial, epithelial and neural cells. CD138 is thought to act primarily as a receptor that modulates cell proliferation, cell migration and cell-matrix associations by linking the extracellular matrix to the cytoskeleton. Heparin sulfate and chondroitin sulfate moieties attached to CD138 associate with several proteins, including collagens, fibronectin, tenascin, thrombospondin and certain cytokines.
Subtype
Primary Antibodies
Target Antigen
CD138 (Syndecan-1)
Alternative Names
B-B4, SDC1, syndecan-1
Reactive Species
Mouse
Conjugation
APC, Biotin, PE, Unconjugated
Host Species
Rat
Cell Type
B Cells, Plasma
Species
Mouse
Application
Cell Isolation, Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Cancer Research, Immunology
Clone
281-2
Gene ID
20969
Isotype
IgG2a, kappa
数据及文献

Data

Data for Unconjugated

Figure 1. Data for Unconjugated

Flow cytometry analysis of Sp2/0 mouse myeloma cells labeled with Anti-Mouse CD138 Antibody, Clone 281-2, followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (Catalog #60138FI) (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 (Catalog #60076), followed by Goat Anti-Mouse IgG (H+L) Antibody, Polyclonal, FITC (solid line histogram).

Data for APC-Conjugated

Figure 2. Data for APC-Conjugated

(A) Flow cytometry analysis of Sp2/0 mouse myeloma cells labeled with Anti-Mouse CD138 Antibody, Clone 281-2, APC (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC (Catalog #60076AZ) (solid line histogram). (B) Flow cytometry analysis of naïve C57BL/6 mouse splenocytes labeled with Anti-Mouse CD138 Antibody, Clone 281-2, APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC (Catalog #60019FI). (C) Flow cytometry analysis of naïve C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC.

Data for Biotin-Conjugated

Figure 3. Data for Biotin-Conjugated

(A) Flow cytometry analysis of Sp2/0 mouse myeloma cells labeled with Anti-Mouse CD138 Antibody, Clone 281-2, Biotin, followed by streptavidin (SAV) APC (filled histogram), or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin (Catalog #60076BT), followed by SAV APC (solid line histogram). (B) Flow cytometry analysis of naïve C57BL/6 mouse splenocytes labeled with Anti-Mouse CD138 Antibody, Clone 281-2, Biotin, followed by SAV APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC (Catalog #60019FI). (C) Flow cytometry analysis of naïve C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, Biotin, followed by SAV APC and Anti-Mouse CD45R Antibody, Clone RA3-6B2, FITC.

Data for PE-Conjugated

Figure 4. Data for PE-Conjugated

(A) Flow cytometry analysis of Sp2/0 mouse myeloma cells labeled with Anti-Mouse CD138 Antibody, Clone 281-2, PE (filled histogram) or Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, PE (Catalog #60076PE) (solid line histogram). (B) Flow cytometry analysis of naïve C57BL/6 mouse splenocytes labeled with Anti-Mouse CD138 Antibody, Clone 281-2, PE and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC (Catalog #60019AZ). (C) Flow cytometry analysis of naïve C57BL/6 mouse splenocytes labeled with Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758, PE and Anti-Mouse CD45R Antibody, Clone RA3-6B2, APC.

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