Anti-Dextran Antibody, Clone DX1

Mouse monoclonal IgG1 antibody against dextran
概要
The DX1 antibody reacts with dextran, a natural polysaccharide consisting of linear chains of alpha-1,6-linked D-glucopyranose residues with short side chains (mostly 1 - 2 glucose units) 1,3- or 1,4-linked to the backbone of the biopolymer. Dextran has several uses as an additive in food, in lacquers, as a plasma volume expander, and as a coating for particles used in bioimaging or cell separation applications. The DX1 antibody was raised against isomaltotetraose (four sugar units in length) but binds better to longer polymer chains. It is also known to bind to amino-dextran, though with lower affinity. This antibody can be used to detect cells labelled with dextran-coated nanoparticles during StemSep™ and EasySep™ cell separation procedures or for other applications that require the detection of dextran.

This antibody clone has been verified for purity assessments of cells isolated with EasySep™ kits, including EasySep™ Human T Cell Enrichment Kit (Catalog #19051) and EasySep™ Human CD4+ T Cell Enrichment Kit (Catalog #19052).
Subtype
一抗
Target Antigen
葡聚糖
Alternative Names
Not applicable
Conjugation
未结合
Host Species
小鼠
Cell Type
Other
Species
人, 小鼠, 大鼠, 非人灵长类动物, 其他
Application
ELISA, 免疫细胞化学, 免疫荧光, 功能检测, 流式细胞术, 细胞分离
Area of Interest
免疫学
Clone
DX1
Gene ID
Not applicable
Isotype
IgG1, kappa
技术资料
Document Type 产品名称 Catalog # Lot # 语言
Product Information Sheet
产品名称
Anti-Dextran Antibody, Clone DX1
60026 All
语言
English
Product Information Sheet
产品名称
Anti-Dextran Antibody, Clone DX1, FITC
60026FI, 60026FI.1 All
语言
English
Safety Data Sheet
产品名称
Anti-Dextran Antibody, Clone DX1
60026 全部
语言
English
Safety Data Sheet
产品名称
Anti-Dextran Antibody, Clone DX1, FITC
60026FI, 60026FI.1 全部
语言
English
数据及文献

数据

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Competitive ELISA showing effect of free dextran (filled circles) or sucrose (empty circles) on the binding of Anti-Dextran Antibody, Clone DX1 to immobilized dextran. (B) Western blot analysis of a dextran-conjugated carrier protein with Anti-Dextran Antibody, Clone DX1. Lane 1, unconjugated carrier protein (negative control); lane 2, carrier protein after conjugation to dextran.

Data for FITC-Conjugated

Figure 2. Data for FITC-Conjugated

Flow cytometry analysis of C57BL/6 mouse bone marrow cells processed with the EasySep™ Mouse CD11b Positive Selection Kit (Catalog #18770) and labeled with Anti-Dextran, Clone DX1, FITC (filled histogram) or a mouse IgG1, kappa FITC isotype control antibody (open histogram).

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