Anti-Human CD63 Antibody, Clone H5C6

Mouse monoclonal antibody against human, rhesus, cynomolgus CD63, unconjugated
概要
The H5C6 antibody reacts with an extracellular epitope on human CD63, a 30-60 kDa type III lysosomal glycoprotein and member of the tetraspanin family. CD63 exhibits broad expression, including on the surface of monocytes, macrophages, activated basophils, fibroblasts, smooth muscle cells, and activated platelets. CD63 is found in late endosomes, lysosomes, and secretory vesicles, and cycles among these compartments. It is also a marker characteristic of extracellular vesicles.

Tetraspanins like CD63 contain four transmembrane domains, two extracellular loops, and short cytoplasmic N- and C-termini. CD63 associates with several integrins, co-receptors, and other proteins to form multimolecular complexes in the plasma membrane called tetraspanin-enriched microdomains. The protein is involved in several cellular processes, including cell activation, adhesion, differentiation, and tumor invasion. CD63 has been implicated in tumor progression, and a deficiency of the protein is associated with Hermansky-Pudlak syndrome, a rare autosomal recessive disorder presenting with platelet dysfunction and defects in lysosomal storage.
Subtype
Primary Antibodies
Target Antigen
CD63
Alternative Names
gp55
Reactive Species
African Green Monkey, Baboon, Capuchin Monkey, Cynomolgus, Rhesus, Chimpanzee, Human
Conjugation
Unconjugated
Host Species
Mouse
Cell Type
Other
Application
Flow Cytometry, Western Blotting
Area of Interest
Extracellular Vesicle Research
Clone
H5C6
Gene ID
967
Isotype
IgG1, kappa
技术资料
Document Type 产品名称 Catalog # Lot # 语言
Product Information Sheet Anti-Human CD63 Antibody, Clone H5C6 100-0139 All English
Special Protocol Anti-Human CD63 Antibody, Clone H5C6 100-0139 All English
Safety Data Sheet Anti-Human CD63 Antibody, Clone H5C6 100-0139 All English
数据及文献

Data

Data showing western blot analysis of extracellular vesicles isolated from mesenchymal stromal cell-conditioned medium with Anti-Human CD63 Antibody, Clone H5C6 and flow cytometry analysis of human peripheral blood mononuclear cells labeled with Anti-Human CD63 Antibody, Clone H5C6 .

Figure 1. Analysis of Anti-Human CD63 Antibody, Clone H5C6 with Western Blot and Flow Cytometry

Extracellular vesicles (EVs) were isolated from mesenchymal stromal cell (MSC)-conditioned medium using a 2 mL EV size exclusion chromatography column and analysed with (A) western blot analysis using Anti-Human CD63 Antibody, Clone H5C6. Lanes (left to right) were loaded with isolated fractions 9, 10, and 11, respectively. Further validation was performed by flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs; viable lymphocytes gated for analysis) stimulated with phorbol myristate acetate (PMA) and ionomycin, then (B) labeled with Anti-Human CD63 Antibody, Clone H5C6, followed by a goat anti-mouse IgG1 antibody, APC (filled histogram), or Anti-Dextran Antibody, Clone DX1 (Catalog #60026) as an isotype control, followed by a goat anti-mouse IgG1 antibody, APC (solid line histogram), or (C) labeled with Anti-Dextran Antibody, Clone DX1 (Catalog #60026) as an isotype control, followed by a goat anti-mouse IgG1 antibody, APC, or (D) labeled with Anti-Human CD63 Antibody, Clone H5C6, followed by a goat anti-mouse IgG1 antibody, APC.

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