Anti-Beta-Tubulin III Antibody, Clone AA10

Mouse monoclonal IgG2a antibody against human, mouse, rat betatubulin III

概要技术资料数据及文献

概要

The AA10 antibody reacts with beta-tubulin III, an ~50 - 55 kDa structural protein that is a constituent of tubulin. Tubulin is the major component of microtubules within the cytoskeleton and is assembled from heterodimers of alpha and beta tubulin subunits. The beta III isoform of tubulin, also known as neuron-specific class III beta-tubulin, is expressed primarily in neurons and is widely used as a marker to distinguish neurons from other cell types. Beta-tubulin III contributes to microtubule formation in neuronal cell bodies and axons, a function involving GTP binding, and plays roles in axonal transport, neuronal cell proliferation, and differentiation. It is highly expressed in several types of cancer and is a predictive and prognostic marker for various tumors, for example, being found in neoplastic but not in normal glial cells. The AA10 antibody is expected to recognize all mammalian homologs of beta-tubulin III.

一抗

β-III微管蛋白

Class 3 beta-tubulin, class III beta-tubulin, MC1R, neuron-specific class 3 beta-tubulin, neuron-specific class III beta-tubulin, TUBB 3, TUBB3, tubulin beta 3, tubulin beta 4, tubulin beta III

人, 小鼠, 大鼠

Alexa Fluor 488, 未结合

小鼠

PSC衍生神经细胞, 神经元

人, 小鼠, 大鼠

Western印迹法, 免疫沉淀, 免疫组化, 免疫细胞化学, 免疫荧光, 流式细胞术

神经科学

AA10

10381

IgG2a, kappa

技术资料

Document Type	产品名称	Catalog #	Lot #	语言
Product Information Sheet	产品名称 Anti-Beta-Tubulin III Antibody, Clone AA10	60100, 60100.1	All	语言 English
Product Information Sheet	产品名称 Anti-Beta-Tubulin III Antibody, Clone AA10, Alexa Fluor® 488	60100AD, 60100AD.1	All	语言 English
Safety Data Sheet	产品名称 Anti-Beta-Tubulin III Antibody, Clone AA10	60100, 60100.1	全部	语言 English
Safety Data Sheet	产品名称 Anti-Beta-Tubulin III Antibody, Clone AA10, Alexa Fluor® 488	60100AD, 60100AD.1	全部	语言 English

数据及文献

数据

Figure 1. Data for Unconjugated

(A) E18 cortical rat neurons were cultured using the NeuroCult™ SM1 Neuronal Culture Kit on poly-lysine-coated glass coverslips, then fixed and labeled with Anti-Beta-Tubulin III Antibody, Clone AA10, followed by donkey anti-mouse IgG, Alexa Fluor® 488, and counter-stained with DAPI. Inset shows cells labeled with Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173 (Catalog #60071), followed by donkey anti-mouse IgG, Alexa Fluor® 488, and counter-stained with DAPI. (B) Western blot analysis of denatured/reduced cell lysates from adult rat brain cortical cells (lane 1) or mouse E13.5 neural progenitor cells cultured with NeuroCult™ Proliferation Kit (Mouse) (negative control, lane 2) with Anti-Beta-Tubulin III Antibody, Clone AA10.

Figure 2. Data for Alexa Fluor® 488-Conjugated

E18 cortical rat neurons were cultured using the NeuroCult™ SM1 Neuronal Culture Kit on poly-lysine-coated glass coverslips, then fixed and labeled with Anti-Beta-Tubulin III Antibody, Clone AA10, Alexa Fluor® 488 (green), and counterstained with DAPI (blue). Inset shows cells incubated with a mouse IgG2a, kappa Alexa Fluor® 488 isotype control antibody, and counterstained with DAPI.