Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21

Mouse monoclonal IgG1, kappa isotype control antibody
概要
The MOPC-21 antibody (IgG1, kappa) is suitable for use as an isotype-matched control antibody in several applications to estimate the degree of non-specific binding by an antigen-specific antibody. Ideally, the isotype control should have the same subclass of heavy chain (IgA, IgD, IgE, IgG, or IgM) and light chain (kappa or lambda) as the specific antibody being used. If a conjugated antibody is used, an isotype control conjugated to the same molecule (e.g. fluorochrome) should be chosen. The use of an appropriate isotype control helps confirm the specificity of the antigen-specific antibody and indicates non-specific binding that may result from binding to Fc receptors or other cell components.

This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).
Subtype
Isotype Controls
Target Antigen
IgG1 Isotype Control
Alternative Names
Not applicable
Conjugation
Alexa Fluor 488, APC, FITC, PE, Unconjugated
Host Species
Mouse
Cell Type
Other
Species
Human, Mouse, Rat, Non-Human Primate, Other
Application
Flow Cytometry, Functional Assay, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blotting
Area of Interest
Immunology
Clone
MOPC-21
Gene ID
Not applicable
Isotype
IgG1, kappa
技术资料
Document Type 产品名称 Catalog # Lot # 语言
Product Information Sheet Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 60070, 60070.1 All English
Product Information Sheet Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor® 488 60070AD, 60070AD.1 All English
Product Information Sheet Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC 60070AZ, 60070AZ.1 All English
Product Information Sheet Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Biotin 60070BT, 60070BT.1 All English
Product Information Sheet Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC 60070FI, 60070FI.1 All English
Product Information Sheet Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE 60070PE, 60070PE.1 All English
Product Information Sheet Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PerCP-Cy5.5 60070PS All English
Safety Data Sheet Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 60070, 60070.1 All English
Safety Data Sheet Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor® 488 60070AD, 60070AD.1 All English
Safety Data Sheet Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC 60070AZ, 60070AZ.1 All English
Safety Data Sheet Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Biotin 60070BT, 60070BT.1 All English
Safety Data Sheet Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC 60070FI, 60070FI.1 All English
Safety Data Sheet Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE 60070PE, 60070PE.1 All English
Safety Data Sheet Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PerCP-Cy5.5 60070PS All English
数据及文献

Data

Data for Unconjugated

Figure 1. Data for Unconjugated

(A) Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, followed by a rat anti-mouse IgG1 antibody, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, Catalog #60107) followed by a rat anti-mouse IgG1 antibody, PE.(B) Human embryonic stem (ES) cells were cultured using mTeSR™1 (Catalog #05850) on Corning® Matrigel®-coated glass slides, then fixed and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, followed by goat anti-mouse IgG, FITC (inset) or with a positive control antibody of the same isotype, Anti-Human SSEA-5 Antibody, Clone 8e11 (Catalog #60063), followed by goat anti-mouse IgG, FITC (green).

Data for Alexa Fluor® 488-Conjugated

Figure 2. Data for Alexa Fluor® 488-Conjugated

(A) Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor® 488 (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, Alexa Fluor® 488, Catalog #60107AD). (B) Human induced pluripotent stem (iPS) cells were cultured using mTeSR™1 (Catalog #05850) on Corning® Matrigel®-coated glass slides, then fixed and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Alexa Fluor® 488 (inset) or with a positive control antibody of the same isotype, Anti-Human SSEA-5 Antibody, Clone 8e11, Alexa Fluor® 488 (Catalog #60063AD, green).

Data for APC-Conjugated

Figure 3. Data for APC-Conjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, APC (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, APC, Catalog #60107AZ).

Data for Biotin-Conjugated

Figure 4. Data for Biotin-Conjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, Biotin followed by streptavidin (SAV) APC (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, Biotin, Catalog #60107BT) followed by SAV APC.

Data for FITC-Conjugated

Figure 5. Data for FITC-Conjugated

Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, FITC (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, FITC, Catalog #60107FI).

Data for PE-Conjugated

Figure 6. Data for PE-Conjugated

(A) Flow cytometry analysis of dendritic cells derived from human peripheral blood mononuclear cells (PBMCs). Cells were processed with the EasySep™ Human CD14 Positive Selection Kit (Catalog #18058) and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD83 Antibody, Clone HB15e, PE, Catalog #60107PE). (B) Human induced pluripotent stem (iPS) cells were cultured using mTeSR™1 (Catalog #05850) on Corning® Matrigel®-coated glass slides, then fixed and labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21, PE (inset) or with a positive control antibody of the same isotype, Anti-Human SSEA-5 Antibody, Clone 8e11, PE (Catalog #60063PE, red).

Data for PE-Conjugated

Figure 7. Data for PS-Conjugated

Flow cytometry analysis of human peripheral blood mononuclear cells (PBMCs) labeled with Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC- 21, PerCP-Cy5.5 (solid line histogram). Filled histogram shows labeling with a mouse IgG1, kappa positive control antibody (Anti-Human CD45 Antibody, Clone HI30, PerCP-Cy5.5; Catalog #60018PS).

Publications (1)

Scientific reports 2014 NOV Trend of telomerase activity change during human iPSC self-renewal and differentiation revealed by a quartz crystal microbalance based assay. Zhou Y et al.
Abstract
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Abstract

Telomerase plays an important role in governing the life span of cells for its capacity to extend telomeres. As high activity of telomerase has been found in stem cells and cancer cells specifically, various methods have been developed for the evaluation of telomerase activity. To overcome the time-consuming procedures and complicated manipulations of existing methods, we developed a novel method named Telomeric Repeat Elongation Assay based on Quartz crystal microbalance (TREAQ) to monitor telomerase activity during the self-renewal and differentiation of human induced pluripotent stem cells (hiPSCs). TREAQ results indicated hiPSCs possess invariable telomerase activity for 11 passages on Matrigel and a steady decline of telomerase activity when differentiated for different periods, which is confirmed with existing golden standard method. The pluripotency of hiPSCs during differentiation could be estimated through monitoring telomerase activity and compared with the expression levels of markers of pluripotency gene via quantitative real time PCR. Regular assessment for factors associated with pluripotency or stemness was expensive and requires excessive sample consuming, thus TREAQ could be a promising alternative technology for routine monitoring of telomerase activity and estimate the pluripotency of stem cells.
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