EasySep™ Serology Whole Blood CD3 Positive Selection Kit

Figure 1. EasySep™ Serology Whole Blood CD3 Positive Selection Kit

Starting with human whole blood, the CD3+ cell content of the isolated fraction typically ranges from 96.7 - 99.6% (note: as the CD3 antibody used for flow cytometry staining was fully blocked by the CD3 antibody used to isolate the cells, staining of T cells was assessed by labeling with anti-CD2; gated on CD45+). In the above example, the purities of the start and final isolated fractions are 25.4% and 98.7% (gated on CD45+), respectively.
NOTE: Red blood cells were removed by lysis prior to flow cytometry.
NOTE: The magnetic particles present on the surface of the isolated CD3+ cells will cause a large FSC/SSC shift when the samples are run on a flow cytometer. This will make it difficult to gate on a specific lymphocyte population based on FSC/SSC. Instead, we recommend first gating the cells on CD45 and examining the lymphocyte populations based on the CD45+ gate.